Human Apolipoprotein AII ELISA Kit (ab184859)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 6 pg/ml
- Range: 31.25 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Apolipoprotein AII ELISA Kit
Apolipoprotein A II/ApoA-II キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% HeLa extract 5 2% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% HeLa extract 3 10.6% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
6 pg/ml -
検出範囲
31.25 pg/ml - 2000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Urine 90.2 89.3% - 90.8% Cell culture media 125.2 122.2% - 127.8% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human Apolipoprotein A II ELISA kit (ab184859) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Apolipoprotein A II protein in human serum, plasma, urine, cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Apolipoprotein A II with 6 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
特記事項
Apolipoprotein AII may stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. ApoA2 forms a disulfide-linked homodimer. Apolipoprotein AII also forms a disulfide-linked heterodimer with APOD and interacts with HCV core protein, APOA1BP and NDRG1.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 10 x 96 tests 10X Human Apolipoprotein AII Capture Antibody 1 x 600µl 1 x 6000µl 10X Human Apolipoprotein AII Detector Antibody 1 x 600µl 1 x 6000µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 120ml Antibody Diluent 5BI 1 x 6ml 10 x 6ml Human Apolipoprotein AII Lyophilized Purified Protein 2 vials 2 x 10 vials Plate Seal 1 unit 1 x 10 units Sample Diluent NS (ab193972) 1 x 50ml 2 x 250ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 x 10 units Stop Solution 1 x 12ml 1 x 120ml TMB Development Solution 1 x 12ml 1 x 120ml -
研究分野
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
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機能
May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. -
組織特異性
Plasma; synthesized in the liver and intestine. -
配列類似性
Belongs to the apolipoprotein A2 family. -
翻訳後修飾
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted. - Information by UniProt
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別名
- APO A2
- Apo AII
- Apo-AII
see all -
参照データベース
- Entrez Gene: 336 Human
- Omim: 107670 Human
- SwissProt: P02652 Human
- Unigene: 237658 Human
関連製品
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Alternative Versions
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ELISA kits
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184859の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Other - Human Apolipoprotein AII ELISA Kit (ab184859)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of ApoA2 standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of Human plasma-EDTA within the working range of the assayBackground-subtracted data values (mean +/- SD) are graphed.
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ApoA2 concentrations in 10 individual Human serum donors.100,000 X diluted serum samples from 10 apparently healthy male donors were measured using this kit. Interpolated data values corrected for sample dilution are graphed in mg of ApoA2 per mL of serum (mean +/- SD, n=3). The mean of ApoA2 concentration of these ten individual serum samples was determined to be 209 μg/mL with a range of 143 – 225 μg/mL.
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Sandwich ELISA - Human Apolipoprotein AII ELISA Kit (ab184859)To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab184859 は論文での使用が確認できていません。