AB318930

Human AMH ELISA Kit

Recombinant
SimpleStep
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Human AMH ELISA Kit is a single-wash 90-min Simplestep used to quantify Human AMH with a sensitivity of 18.389 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput

別名を表示する

MIF, AMH, Anti-Muellerian hormone, Muellerian-inhibiting factor, Muellerian-inhibiting substance, MIS

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Sandwich ELISA - Human AMH ELISA Kit (AB318930)

sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Interpolated concentrations of human AMH in human serum in 96-well vs. 384-well plates. Interpolated concentration of native AMH was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 22.5% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Interpolated concentrations of AMH in normal human serum donors. Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AMH concentration of detected samples was determined to be 2,090 pg/mL with a range of undetected – 6,208 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Interpolated concentrations of AMH in normal human serum donors. Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean AMH concentration was determined to be 2,698 pg/mL with a range of 998 – 4,674 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Interpolated concentrations of human AMH in serum, plasma (citrate), plasma (EDTA), and plasma (heparin). Interpolated concentration of native AMH was measured in duplicate at different sample concentrations. Undiluted samples are 45% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Interpolated concentrations of human AMH in monkey serum and rat serum. Interpolated concentration of native AMH was measured in duplicate at different sample concentrations. Undiluted samples are 22.5% rat serum, and 2.25% monkey serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Example of human AMH standard curve in Sample Diluent NS + 1X Enhancer in 96-well vs. 384-well plate. Example of human AMH standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human AMH ELISA Kit (AB318930)

Example of human AMH standard curve in Sample Diluent NS + 1X Enhancer. Example of human AMH standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, EDTA Plasma, Cell culture media, Citrate plasma, Heparin Plasma

交差種

Human, Rat, Monkey

アッセイタイプ

Sandwich

Quantitative

検出感度

= 18.389 pg/mL

検出範囲

43.75 - 2800 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Human AMH SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of AMH protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human AMH with 18.389 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)
-Also available in a fully validated 384-well format.

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.6" } ]

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回収率

[ { "sample": "Serum", "range": "87 - 108 %", "average": "= 100" }, { "sample": "Citrate plasma", "range": "100 - 117 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "94 - 105 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "86 - 105 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "82 - 106 %", "average": "= 98" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

The anti-Muellerian hormone (AMH) plays an important role in several reproductive functions (PubMed : 14742691, PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). Anti-Muellerian hormone binds and activates AMHR2, its specific type-II receptor, that heterodimerizes with type-I receptors (ACVR1 and BMPR1A) to regulate target gene expression through downstream SMAD protein signal transduction (PubMed : 20861221, PubMed : 34155118). Produced and secreted by Sertoli cells of the male fetus, anti-Muellerian hormone induces Muellerian duct regression during male fetal sexual differentiation (PubMed : 34155118, PubMed : 3754790, PubMed : 8469238). In female, it is produced by granulosa cells of the preantral and small antral follicles and acts as a negative regulator of the primordial to primary follicle transition and decreases FSH sensitivity of growing follicles (PubMed : 14742691). Also plays a role in Leydig cell differentiation and function (By similarity).

See full target information AMH

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websiteProtocolBooklet

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Human AMH ELISA Kit