Human alpha Fetoprotein ELISA Kit (AFP) (ab108838)
Key features and details
- Sensitivity: 0.38 ng/ml
- Range: 0.625 ng/ml - 40 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human alpha Fetoprotein ELISA Kit (AFP)
alpha 1 Fetoprotein キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.1% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10.5% -
サンプルの種類
Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.38 ng/ml -
検出範囲
0.625 ng/ml - 40 ng/ml -
添加回収試験
104 %
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全工程の試験時間
4h 0m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s alpha Fetoprotein (AFP) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of alpha Fetoprotein levels in plasma, serum, cell lysate and culture supernatants, and tissue samples.
An alpha Fetoprotein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an alpha Fetoprotein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of alpha Fetoprotein captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human alpha Fetoprotein Antibody 1 x 120µl alpha Fetoprotein Microplate (12 x 8 well strips) 1 unit alpha Fetoprotein Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
組織特異性
Plasma. Synthesized by the fetal liver and yolk sac. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
発生段階
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻訳後修飾
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Afp
- AFPD
- Alpha fetoglobulin
see all -
参照データベース
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108838の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve
Representative Standard Curve using ab108838.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab108838 は 11 報の論文で使用されています。
- Zabady S et al. Cyanidin-3-Glucoside Modulates hsa_circ_0001345/miRNA106b/ATG16L1 Axis Expression as a Potential Protective Mechanism against Hepatocellular Carcinoma. Curr Issues Mol Biol 44:1677-1687 (2022). PubMed: 35723373
- Carbonaro M et al. Efficient engraftment and viral transduction of human hepatocytes in an FRG rat liver humanization model. Sci Rep 12:14079 (2022). PubMed: 35982097
- Aboughaleb IH et al. Integration of transcriptomes analysis with spectral signature of total RNA for generation of affordable remote sensing of Hepatocellular carcinoma in serum clinical specimens. Heliyon 7:e06388 (2021). PubMed: 33748469
- Kats D et al. Volasertib preclinical activity in high-risk hepatoblastoma. Oncotarget 10:6403-6417 (2019). PubMed: 31741706
- Lv S et al. H2-Based Electrochemical Biosensor with Pd Nanowires@ZIF-67 Molecular Sieve Bilayered Sensing Interface for Immunoassay. Anal Chem 91:12055-12062 (2019). PubMed: 31436433