Human Albumin ELISA Kit (ab108788)
Key features and details
- Sensitivity: 0.79 ng/ml
- Range: 3.125 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cerebral Spinal Fluid, Milk, Saliva, Tissue, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Albumin ELISA Kit
Albumin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 5.9% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 10.4% -
サンプルの種類
Cell culture supernatant, Saliva, Milk, Urine, Tissue, Cell Lysate, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.79 ng/ml -
検出範囲
3.125 ng/ml - 200 ng/ml -
添加回収試験
99 %
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全工程の試験時間
3h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Human Albumin ELISA kit (ab108788) is a sandwich ELISA kit designed for the quantitative measurement of albumin levels in urine, saliva, milk, CSF, cell culture supernatants, cell lysate and tissue samples.
An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.
Get results in 90 minutes with Human Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.
Please note: For both the 10X Diluent N Concentrate and 20X Wash Buffer Concentrate, please ensure to mix well by inversion before use to avoid precipitation.
The concentration of the biotinylated antibody varies by lot: 30X, 40X, 50X, etc. The stock Biotinylated Albumin Antibody must be diluted with 1X Diluent N to prepare 1X Biotinylated Albumin Antibody for use in the assay procedure. Observe the label for the “X” concentration on the vial of Biotinylated Albumin Antibody.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 25X Biotinylated Human Albumin Antibody 1 x 200µl Albumin Microplate (12 x 8 well strips) 1 unit Albumin Standard 1 vial Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. -
組織特異性
Plasma. -
関連疾患
Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population. -
配列類似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
翻訳後修飾
Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
細胞内局在
Secreted. - Information by UniProt
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別名
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
参照データベース
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
関連製品
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SimpleStep ELISA kits
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108788の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Typical Standard Curve for ab108788 Human Albumin ELISA kit
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ab108788 Albumin Human ELISA kitStandard curve with background signal subtracted (duplicates; +/- SD).
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ab108788 Albumin Human ELISA kitConcentration of albumin from differently diluted (in parenthesis) samples, demonstrating a linearity of 84-90% in CSF and 88-111% in milk (duplicates; +/- SD).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (55)
ab108788 は 55 報の論文で使用されています。
- Carbonaro M et al. IL-6-GP130 signaling protects human hepatocytes against lipid droplet accumulation in humanized liver models. Sci Adv 9:eadf4490 (2023). PubMed: 37058568
- Jin Y et al. Adipose-derived stem cells show hepatic differentiation potential and therapeutic effect in rats with acute liver failure. Acta Biochim Biophys Sin (Shanghai) 55:601-612 (2023). PubMed: 37078751
- Wilton DK et al. Microglia and complement mediate early corticostriatal synapse loss and cognitive dysfunction in Huntington's disease. Nat Med 29:2866-2884 (2023). PubMed: 37814059
- Cayley AN et al. Development of a network of carcinogenicity adverse outcome pathways and its employment as an evidence framework for safety assessment. ALTEX N/A:N/A (2022). PubMed: 35575642
- Daley MC et al. Beyond pharmaceuticals: Fit-for-purpose new approach methodologies for environmental cardiotoxicity testing. ALTEX N/A:N/A (2022). PubMed: 35648122