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AB324511

Human Aggrecan ELISA Kit

Human Aggrecan ELISA Kit

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Human Aggrecan ELISA Kit is a Sandwich ELISA for the measurement of Human Aggrecan in Human in Biofluids, Other samples samples.

別名を表示する

AGC1, CSPG1, MSK16, ACAN, Aggrecan core protein, Cartilage-specific proteoglycan core protein, Chondroitin sulfate proteoglycan core protein 1, CSPCP, Chondroitin sulfate proteoglycan 1

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Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)
  • sELISA

Lab

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)

Interpolated concentration of native Aggrecan was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 20% serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)
  • sELISA

Lab

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)

Interpolated concentration of native Aggrecan was measured in duplicate at different sample concentrations. Undiluted samples are serum 20%, plasma (citrate) 20%, plasma (EDTA) 20%, plasma (heparin) 20%, and synovial fluid 2%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)
  • sELISA

Lab

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)

Example of human Aggrecan standard curve in Sample Diluent NS in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)
  • sELISA

Supplier Data

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)

Interpolated concentration of native Aggrecan was measured in duplicate at different sample concentrations. Undiluted samples are urine 50%, and A204 cell culture supernatant 100%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)
  • sELISA

Supplier Data

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Aggrecan concentration was determined to be 26.1 ng/mL with a range of 9.65 – 39.33 ng/mL.

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)
  • sELISA

Lab

Sandwich ELISA - Human Aggrecan ELISA Kit (AB324511)

Example of human Aggrecan standard curve in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Citrate plasma, Heparin Plasma, EDTA Plasma, Supernatant, Urine, Other biological fluids

交差種

Human

アッセイタイプ

Sandwich

検出感度

= 13.44 pg/mL

検出範囲

78.125 - 5000 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Human Aggrecan ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Aggrecan with a sensitivity of 13.44pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Design your own immunoassay: we also offer the conjugation-ready antibody pair.

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精度

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.6" } ]
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回収率

[ { "sample": "Serum", "range": "103 - 105 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "103 - 110 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "99 - 103 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "105 - 109 %", "average": "= 107" }, { "sample": "Cell culture media", "range": "80 - 100 %", "average": "= 90" }, { "sample": "Urine", "range": "98 - 112 %", "average": "= 103" }, { "sample": "Other biological fluids", "range": "78 - 84 %", "average": "= 81" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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製品プロトコール

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ターゲットの情報

This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.
See full target information ACAN
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websiteProtocolBooklet
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