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リコンビナントSimpleStep

GST His-tag ELISA Kit (ab302758)

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Interpolated concentrations of spiked species GST His-tag in serum, lauryl maltoside buffer, urea buffer, 10F RPMI, and Guanidine buffer.
  • Example of species GST His-tag standard curve in 1X Cell Extraction Buffer PTR.
  • Simple Step Elisa Protocol
  • Benefits of Recombinant Antibodies

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.476 pg/ml
  • Range: 35.156 pg/ml - 2250 pg/ml
  • Sample type: Cell culture media, Cell culture supernatant
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)

製品の概要

  • 製品名

    GST His-tag ELISA Kit

  • 検出方法

    Colorimetric

  • 再現性

    Intra-Assay（同時再現性）
    サンプル N 平均値 SD CV%
    Supernatant 8 7.8%
    Inter-Assay（日差再現性）
    サンプル N 平均値 SD CV%
    Supernatant 3 9.7%

  • サンプルの種類

    Cell culture supernatant, Cell culture media

  • アッセイタイプ

    Sandwich (quantitative)

  • 検出感度

    3.476 pg/ml

  • 検出範囲

    35.156 pg/ml - 2250 pg/ml

  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 108 92% - 120%
    Cell culture media 91 81% - 110%

  • 全工程の試験時間

    1h 30m

  • ステップ

    One step assay

  • 製品の概要

    GST His-tag SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GST His-tag protein. Quantitate GST His-tag with 3.476 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


    -Single-wash protocol reduces assay time to 90 minutes or less
    -High sensitivity, specificity and reproducibility from superior antibodies
    -Fully validated in biological samples
    -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • 特記事項

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • アプリケーション

    適用あり: Sandwich ELISAmore details

  • 試験プラットフォーム

    Pre-coated microplate (12 x 8 well strips)

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab302758の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Sandwich ELISA
Use at an assay dependent concentration.
特記事項
Sandwich ELISA
Use at an assay dependent concentration.

画像

  • Interpolated concentrations of spiked species GST His-tag in serum, lauryl maltoside buffer, urea buffer, 10F RPMI, and Guanidine buffer.
    Interpolated concentrations of spiked species GST His-tag in serum, lauryl maltoside buffer, urea buffer, 10F RPMI, and Guanidine buffer.

    Recombinant GST His-tag was spiked into the following samples and diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. Undiluted samples are as follows: serum 10%, lauryl maltoside buffer 50%, urea buffer 10%, 10F RPMI 50%, and guanidine buffer 0.1%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Example of species GST His-tag standard curve in 1X Cell Extraction Buffer PTR.
    Example of species GST His-tag standard curve in 1X Cell Extraction Buffer PTR.

    Example of species GST His-tag standard curve. Background-subtracted data values (mean +/- SD) are graphed.

  • Simple Step Elisa Protocol
    Simple Step Elisa Protocol

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Benefits of Recombinant Antibodies
    Benefits of Recombinant Antibodies

参考文献 (0)

ab302758 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab302758 は論文での使用が確認できていません。

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