AB316916

GFP ELISA Kit- Intracellular

SimpleStep
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GFP ELISA Kit- Intracellular is a single-wash 90-min Simplestep used to quantify GFP with a sensitivity of 2.523 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

See more GFP assay kits and antibodies

別名を表示する

Green fluorescent protein, GFP

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Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)

sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)

Interpolated concentrations of spiked eGFP in Jurkat extract and spiked denatured-eGFP in Jurkat extract

Recombinant untreated eGFP and 2% SDS denatured-eGFP were diluted 1 : 20 in 500 µg/mL Jurkat extract (prepared in 1X Cell extraction Buffer PTR Without Enhancer) and then diluted in a 2-fold dilution series in 1X Cell Extraction Buffer PTR. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)

Interpolated concentrations of GFP in HEK293 overexpressing GFP cell extract

Interpolated concentration of native GFP was measured in duplicate at different sample concentrations. Undiluted samples are 50 ng/mL HEK293 overexpressing GFP cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)

Example of GFP standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - GFP ELISA Kit- Intracellular (AB316916)

Standard curve comparison between the original GFP SimpleStep ELISA (ab171581) and current GFP SimpleStep ELISA (ab316916).

Key facts

検出方法

Colorimetric

サンプルタイプ

Tissue Extracts, Cell Lysate

交差種

Aequorea victoria

アッセイタイプ

Sandwich

Quantitative

検出感度

= 2.523 pg/mL

検出範囲

9.375 - 600 pg/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

GFP SimpleStep ELISA^® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GFP protein in Tissue extracts, Cell Lysate. Quantitate GFP with 2.523 pg/ml sensitivity.

SimpleStep ELISA^® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details. Our SimpleStep ELISA^® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA^® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA^® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" } ]

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回収率

[ { "sample": "Tissue Extracts", "range": "82 - 107 %", "average": "= 93" }, { "sample": "Cell Lysate", "range": "82 - 92 %", "average": "= 86" } ]

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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製品プロトコール

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ターゲットの情報

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

See full target information GFP

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websiteProtocolBooklet

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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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GFP ELISA Kit- Intracellular