AB274408

Dog Thymidine Kinase ELISA Kit (TK1)

Name: Dog Thymidine Kinase ELISA Kit (TK1) (ab274408) | Abcam
Brand: Abcam Limited
SKU: AB274408
Price: 131000 JPY
Availability: InStock

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Dog Thymidine Kinase ELISA Kit (TK1) is a Sandwich (quantitative) ELISA for the measurement of Dog Thymidine Kinase (TK1) in Dog in Biofluids samples.

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Sandwich ELISA - Dog Thymidine Kinase ELISA Kit (TK1) (AB274408)

sELISA

Supplier Data

Sandwich ELISA - Dog Thymidine Kinase ELISA Kit (TK1) (AB274408)

Example standard curve using Canine Thymidine Kinase (TK1) ELISA Kit (ab274408).

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Serum

交差種

Dog

アッセイタイプ

Sandwich (quantitative)

検出範囲

0.16 - 5 ng/mL

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

The Dog Thymidine Kinase ELISA Kit (TK1) (ab274408) is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring TK1 in dog biological samples.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Thymidine Kinase 1 (TK1) also known as Thymidine Kinase isoform A is an enzyme involved in the phosphorylation of thymidine into thymidine monophosphate an important component in DNA synthesis. TK1 has a molecular weight of approximately 25 kDa. It is predominantly expressed in proliferating cells with expression levels peaking during the S phase of the cell cycle. You often find TK1 in tissues with high cell division rates like bone marrow and lymphatic tissues.

Biological function summary

This enzyme is critical for DNA replication and repair by providing deoxythymidine triphosphate (dTTP) a deoxynucleoside triphosphate. TK1 operates as a monomer but can form a tetrameric complex in its active state which enhances its affinity for substrates. TK1 can process various substrates including thymidine and thymidine analogs such as 5-bromo-2'-deoxyuridine (BrdU) and EdU. These substrates are vital in molecular biology as they help in measuring DNA synthesis in cell proliferation studies.

Pathways

The function of TK1 is central to the salvage pathway of nucleotide synthesis. This pathway allows recycling of thymidine from degraded DNA which is particularly important for rapidly dividing cells that need to ensure a sufficient supply of nucleotides. TK1 acts alongside other enzymes such as thymidylate synthase and ribonucleotide reductase to maintain nucleotide balance within the cell ensuring proper DNA synthesis and cellular proliferation.

TK1 levels often increase in cancerous tissues due to their elevated cell proliferation rates. Serum TK1 is a biomarker for various cancers like leukemia and breast cancer. Studies show a correlation between high TK1 activity and tumor aggressiveness and progression. Additionally interactions of TK1 with the protein p53 a tumor suppressor show that defects in TK1 regulation can contribute to genomic instability highlighting its role in oncogenesis.

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製品プロトコール

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ターゲットの情報

See full target information TK1

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websiteProtocolBooklet

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Abcam product promise

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Dog Thymidine Kinase ELISA Kit (TK1)