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Cortisol ELISA Kit (ab154996)

Reviews (2)Q&A (1)References (9)
Typical Standard Curve

    Key features and details

    • Sensitivity: 0.12 ng/ml
    • Range: 0.5 ng/ml - 100 ng/ml
    • Sample type: Saliva
    • Detection method: Colorimetric
    • Assay type: Competitive
    • Reacts with: Human

    製品の概要

    • 製品名

      Cortisol ELISA Kit
      Cortisol キット 製品一覧

    • 検出方法

      Colorimetric

    • 再現性

      Intra-Assay（同時再現性）
      サンプル N 平均値 SD CV%
      Saliva 60 <=10%
      Inter-Assay（日差再現性）
      サンプル N 平均値 SD CV%
      Saliva 30 <=8.3%

    • サンプルの種類

      Saliva

    • アッセイタイプ

      Competitive

    • 検出感度

      0.12 ng/ml

    • 検出範囲

      0.5 ng/ml - 100 ng/ml

    • 添加回収試験

      95.42 %

    • ステップ

      Multiple steps standard assay

    • 種交差性

      交差種: Human

    • 製品の概要

      Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in saliva.


      A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

    • アプリケーション

      適用あり: Competitive ELISAmore details

    • 試験プラットフォーム

      Microplate

    製品の特性

    • 保存方法

      Store at +4°C. Please refer to protocols.
    • 内容 1 x 96 tests
      10X Wash Solution 1 x 50ml
      Anti-Cortisol IgG Coated Microplate (12 x 8 wells) 1 unit
      Control A 1 x 1ml
      Control B 1 x 1ml
      Cortisol Standard 0 - 0 ng/mL 1 x 1ml
      Cortisol Standard 1 - 0.5 ng/mL 1 x 1ml
      Cortisol Standard 2 – 1 ng/mL 1 x 1ml
      Cortisol Standard 3 – 5 ng/mL 1 x 1ml
      Cortisol Standard 4 – 10 ng/mL 1 x 1ml
      Cortisol Standard 5 – 20 ng/mL 1 x 1ml
      Cortisol Standard 6 – 100 ng/mL 1 x 1ml
      Cortisol-HRP Conjugate (Concentrated) 1 x 1ml
      Foil Cover 1 unit
      Incubation Buffer 1 x 30ml
      Stop Solution 1 x 15ml
      Strip holder 1 unit
      TMB Substrate Solution 1 x 15ml

    • 研究分野

    関連製品

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab154996の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Competitive ELISA
    Use at an assay dependent concentration.
    特記事項
    Competitive ELISA
    Use at an assay dependent concentration.

    画像

    • Typical Standard Curve
      Typical Standard Curve

      Representative Standard Curve using ab154996.

    参考文献 (9)

    ab154996 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab154996 は 9 報の論文で使用されています。

    • Sriboonlert J  et al. Cananga odorata Aromatherapy Reduces Anxiety in Unexperienced Patients Hospitalized for Interventional Neuroradiology Procedures: A Randomized Control Trial. J Evid Based Integr Med 28:2515690X221150527 (2023). PubMed: 36659818
    • Davis L  et al. "Following the Breath:" A Trauma-Informed Intervention for Educator Wellness in Rural Montana. Educ Sci (Basel) 13:N/A (2023). PubMed: 38116307
    • Heiland EG  et al. ABBaH teens: Activity Breaks for Brain Health in adolescents: study protocol for a randomized crossover trial. Trials 23:22 (2022). PubMed: 34991692
    • Davis L & Aylward A Mitigating Rural Adolescent Trauma: Remote Delivery of a Trauma-Informed Yoga Intervention During COVID-19. J Child Adolesc Trauma N/A:1-14 (2022). PubMed: 36471891
    • Davis L  et al. Trauma-Informed Yoga: Investigating an Intervention for Mitigating Adverse Childhood Experiences in Rural Contexts. Educ Stud 58:530-559 (2022). PubMed: 36654845
    • Romero-Martínez Á  et al. Hormonal Differences in Intimate Partner Violence Perpetrators When They Cope with Acute Stress: A Pilot Study. Int J Environ Res Public Health 18:N/A (2021). PubMed: 34071628
    • Heiland EG  et al. Frequent, Short Physical Activity Breaks Reduce Prefrontal Cortex Activation but Preserve Working Memory in Middle-Aged Adults: ABBaH Study. Front Hum Neurosci 15:719509 (2021). PubMed: 34602995
    • Heiland EG  et al. ABBaH: Activity Breaks for Brain Health. A Protocol for a Randomized Crossover Trial. Front Hum Neurosci 14:273 (2020). PubMed: 32760263
    • Ruiz-Babot G  et al. Modeling Congenital Adrenal Hyperplasia and Testing Interventions for Adrenal Insufficiency Using Donor-Specific Reprogrammed Cells. Cell Rep 22:1236-1249 (2018). PubMed: 29386111

    レビューと Q&A

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    レビューを送る 質問を送る

    1-3 of 3 Abreviews or Q&A

    Hair sample

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image
    It was easy to use and gave good result.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 23 2019

    Happy with the product

     Good Excellent 5/5 (Ease of Use)
    Abreviews
    abreview image
    We bought this kit mainly because it does not cross react with dexamethasone as stated in the protocol. We used guinea pig saliva and followed the protocol exactly. Overall, kit was easy to use with straight forward instructions. Our curve was good, all samples fell within the range as expected. Only reason not rating this a 5* is that although the kit should not detect dex our own internal controls contradicted this, but only slightly and the dex was a high concentration. Will use this kit again.
    DISCOUNT CODE 190304 001480 A
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MS. Alice Kostaki

    Verified customer

    投稿 Mar 14 2019

    Question

    Thank you for your continuous support ,

    One of our customers is interested in the Cortisol ELISA Kit code # ab154996 to detect cortisol level from Saliva samples.

    Reference to the attached handbook, kindly advise about the below :


    Is there any recommended saliva collection device for this kit, especially for delayed storage of cases for later testing?

    For how long can the end user store the samples before testing and at which conditions?

    Read More
    Answer



    For ab154996 we recommend to collect saliva samples with a centrifuge glass tube and a plastic straw or with the “Salivette” (Sarstedt, Ref. 511534500).

    The following should be noted:
    a) If saliva collection is carried out in the morning ensure that this is carried out prior to brushing teeth
    b) During the day allow 1 hour after any food or drink before collecting saliva samples
    c) It is very important that a good clear sample is received – i.e. no contamination with food, lipstick, blood
    (bleeding gums) or other extraneous materials.


    Processing Instructions
    1. Let the saliva flow down through the straw into the centrifuge glass tube
    2. Centrifuge at 3000 rpm for 15 min.
    3. Store at -20°C for at least 1 hour
    4. Defrost samples
    5. Centrifuge again for 15 min. at 3000 rpm
    6. The saliva sample is now ready to be tested.
    7. Store the sample at 4 °C for one week or at -20°C for longer term storage.


    Saliva Processing Instructions with Salivette Sarstedt
    1. Remove the swab from the suspended insert of the Salivette.
    2. Gently chewing the swab for 1 minute produces a sufficient quantity of Saliva.
    3. Replace the swab into the Salivette and firmly close the tube using the stopper.
    4. Centrifuge the Salivette for 2 minutes at 1000g (rcf) for saliva generation.
    5. Remove the insert complete with the swab from the centrifuge vessel and discard. The clear Saliva is now
    ready for analysis (at least 1 ml of saliva should be recovered with this method).

    For storage of samples we would recommend to freeze them at -20C. We have not tested how long samples can be stored frozen without loosing quality but believe a couple month should not be a problem.

    Read More

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