AB273175

Canine MCP1 ELISA Kit

Name: Canine MCP1 ELISA Kit (ab273175) | Abcam
Brand: Abcam Limited
SKU: AB273175
Price: 109000 JPY
Availability: InStock

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Canine MCP1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Canine MCP1 in Dog in Biofluids, Cell Culture Media samples.

別名を表示する

MCP1, SCYA2, CCL2, C-C motif chemokine 2, Monocyte chemoattractant protein 1, Monocyte chemotactic protein 1, Small-inducible cytokine A2, MCP-1

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Sandwich ELISA - Canine MCP1 ELISA Kit (AB273175)

sELISA

Supplier Data

Sandwich ELISA - Canine MCP1 ELISA Kit (AB273175)

Canine MCP1 ELISA Kit standard curve.

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Cell culture supernatant, Serum

交差種

Dog

アッセイタイプ

Sandwich (quantitative)

検出感度

= 9 pg/mL

検出範囲

10.94 - 700 pg/mL

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Canine MCP1 ELISA Kit (ab273175) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine MCP1 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Canine MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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精度

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]

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回収率

[ { "sample": "Cell culture supernatant", "range": "85 - 114 %", "average": "= 101" }, { "sample": "Serum", "range": "93 - 103 %", "average": "= 96.01" }, { "sample": "Plasma", "range": "76 - 100 %", "average": "= 89.27" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab273175-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB273175-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Canine MCP1 coated Microplate (12 x 8 wells)", "number":"AB273175-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB273175-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB273175-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Canine MCP1 Antibody", "number":"AB273175-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB273175-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Canine MCP1 Standard (Lyophilized)", "number":"AB273175-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB273175-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB273175-CMP06", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.

Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.

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製品プロトコール

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ターゲットの情報

Acts as a ligand for C-C chemokine receptor CCR2 (By similarity). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (By similarity). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity). May play a significant role in monocyte trafficking into the reperfused myocardium (PubMed : 9024159).

See full target information CCL2

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websiteProtocolBooklet

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Canine MCP1 ELISA Kit