AB157700

Canine IgE ELISA Kit

JP Hazardous:医薬用外毒物
JP Deleterious:医薬用外劇物

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(2 Publications)

Canine IgE ELISA Kit is a sandwich ELISA designed to quantify Canine IgE with a sensitivity of 2.2897 ng/mL.

- Colorimetric sandwich ELISA - 450 nm readout - works on any plate reader
- Wide dynamic range - quantifies 6.25 - 400 ng/mL

1 Images

Sandwich ELISA - Canine IgE ELISA Kit (AB157700)

sELISA

Supplier Data

Sandwich ELISA - Canine IgE ELISA Kit (AB157700)

Representative standard curve using ab157700 IgE Dog ELISA kit

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Serum, Other biological fluids

交差種

Dog

アッセイタイプ

Sandwich (quantitative)

検出感度

= 2.2897 ng/mL

検出範囲

6.25 - 400 ng/mL

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Canine IgE ELISA Kit ab157700 is a sandwich ELISA to measure Canine IgE in serum, plasma, other biological fluids with a sensitivity of 2.2897 ng/ml.

How the assay works

In this assay the IgE present in samples reacts with the anti-IgE antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgE antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgE. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgE in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The quantity of IgE in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Canine IgE ELISA Kit ab157700 protocol summary

1. Add standard or sample to each well used. Incubate at room temperature.
2. Aspirate and wash each well.
3. Add prepared HRP labeled secondary detector antibody. Incubate at room temperature
4. Aspirate and wash each well.
5. Add Chromogen Substrate Solution to each well.
6. Immediately begin recording the color development

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精度

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]

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回収率

[ { "sample": "Serum", "range": null, "average": "> 85" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab157700-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Chromogen Substrate Solution", "number":"AB157700-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Dog IgE Calibrator (lyophilized)", "number":"AB157700-CMP01", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Wash Buffer Concentrate", "number":"AB157700-CMP06", "productcode":"" }, { "size":"1 x 150 µL", "name":"100X HRP-conjugated anti-dog IgE antibody", "number":"AB157700-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"5X Diluent Concentrate", "number":"AB157700-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Dog IgE ELISA Microplate", "number":"AB157700-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB157700-CMP03", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

Multi

保管に関する情報

Please refer to protocols

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The IgE protein [also known as immunoglobulin E] is a type of antibody with a molecular mass of approximately 190 kDa. IgE is expressed primarily on the surface of mast cells and basophils. It plays a central role in the immune system by binding to allergens and triggering the release of inflammatory mediators from mast cells and basophils. IgE's high-affinity binding to its receptor FcεRI on these effector cells facilitates this process. IgE's activity is central to initiating immediate hypersensitivity reactions which are a part of the body's immune response.

Biological function summary

The IgE protein functions as a mediator in allergic reactions. It does not operate as part of a complex but directly interacts with allergens and the IgE-specific receptors on immune cells. IgE's role is important in defense against parasitic infections where it performs a protective function by promoting eosinophil activation and degranulation. This action helps the body to expel the parasites. In the absence of parasitic infection IgE's function translates into its role in allergic responses where it becomes responsible for the symptoms associated with allergic diseases.

Pathways

IgE is an important player in the immune response pathway. The protein interacts with allergy-related pathways such as the T helper 2 (Th2) pathway. IgE's role in this pathway involves cooperation with cytokines notably IL-4 and IL-13 which are produced by Th2 cells and promote IgE synthesis in B cells. Also IgE is linked to the FcεRI receptor signaling pathway that initiates the release of histamines and other mediators when IgE binds to an allergen leading to an allergic response.

IgE is closely linked to allergic conditions like asthma and allergic rhinitis. These conditions result from IgE-mediated inflammatory responses that affect the respiratory system. In asthma IgE antibodies play a role in airway inflammation and hyperreactivity by promoting the activation of multiple immune cells including eosinophils and mast cells. IgE's involvement in allergic rhinitis manifests through similar mechanisms with nasal inflammation and irritation. IgE's interaction with other proteins such as histamine-releasing factor exacerbates these conditions by increasing the release of histamine which intensifies the allergic symptoms.

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製品プロトコール

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ターゲットの情報

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

PloS one 17:e0271932 PubMed35867776

2022

Hydrolyzed chicken liver used as single source of animal protein in diet and its effect on cytokines, immunoglobulins, and fecal microbiota profile of adult dogs.

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View all publications

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websiteProtocolBooklet

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Canine IgE ELISA Kit