AB157699

Canine IgA ELISA Kit

JP Hazardous:医薬用外毒物
JP Deleterious:医薬用外劇物

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(2 Publications)

Canine IgA ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Canine IgA in Dog in Biofluids samples.

1 Images

Sandwich ELISA - Canine IgA ELISA Kit (AB157699)

sELISA

Supplier Data

Sandwich ELISA - Canine IgA ELISA Kit (AB157699)

Representative standard curve using ab157699 IgA Dog ELISA Kit

Key facts

検出方法

Colorimetric

サンプルタイプ

Plasma, Serum

交差種

Dog

アッセイタイプ

Sandwich (quantitative)

検出感度

= 7.767 ng/mL

検出範囲

31.25 - 1000 ng/mL

アッセイプラットフォーム

Microplate

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Reactivity data

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製品の詳細

Abcam's IgA Dog ELISA kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for the quantitative measurement of IgA in dog serum and plasma.

In this assay the IgA present in samples reacts with the anti-IgA antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-IgA antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgA. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3',5,5'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgA in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgA in the test sample. The quantity of IgA in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

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製品内容

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

Multi

保管に関する情報

Please refer to protocols

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Immunoglobulin A (IgA) also known as sIgA in its secretory form is an important component of the immune system. With a molecular weight of approximately 160 kDa IgA exists as a monomeric or dimeric structure. It is primarily expressed in mucosal areas such as the respiratory tract gastrointestinal tract and urogenital tract while also being present in secretions like saliva tears and breast milk. In its dimeric form IgA associates with a joining (J) chain and a secretory component which facilitates its transport across mucosal barriers.

Biological function summary

IgA functions as a first line of defense in immune responses occurring at mucosal surfaces. Its primary role is to neutralize pathogens toxins and prevent their attachment and penetration through epithelial cells. This antibody does not form part of a larger complex but plays an important role in immune exclusion by trapping antigens in the mucus layer. Additionally IgA can mediate antibody-dependent cellular cytotoxicity and engage with specific receptors like FcαRI (CD89) on immune cells further enhancing its protective capabilities.

Pathways

IgA participates in the mucosal immune response which is a sub-component of the broader immune system pathways. It is closely associated with the polymeric immunoglobulin receptor (pIgR) which transports IgA to the mucosal surfaces. The pathway involves recombination activation proteins (RAG) during its class-switching process in B cells differentiating from IgM-producing cells to those secreting IgA. These pathways are integral to maintaining the mucosal environment and protecting against pathogens.

Alterations or deficiencies in IgA can lead to increased susceptibility to infections and disorders like IgA nephropathy and celiac disease. In IgA nephropathy IgA deposits in the kidneys result in inflammation and damage to renal tissues. Celiac disease involves an immune reaction to gluten where IgA antibodies against tissue transglutaminase (tTG) contribute to intestinal damage. Both conditions illustrate the importance of IgA in maintaining immune balance and its connection with disease pathologies.

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製品プロトコール

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ターゲットの情報

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Veterinary world 16:834-843 PubMed37235154

2023

Influence of dietary supplementation with new strains on hematology, serum biochemistry, nutritional status, digestibility, enzyme activities, and immunity in dogs.

Applications

Unspecified application

Species

Unspecified reactive species

Kamonporn Panja,Sathita Areerat,Pipatpong Chundang,Pornsucha Palaseweenun,Nattaphong Akrimajirachoote,Jaruwan Sitdhipol,Punnathorn Thaveethaptaikul,Pennapa Chonpathompikunlert,Kanidta Niwasabutra,Pongsathon Phapugrangkul,Attawit Kovitvadhi

PloS one 17:e0271932 PubMed35867776

2022

Hydrolyzed chicken liver used as single source of animal protein in diet and its effect on cytokines, immunoglobulins, and fecal microbiota profile of adult dogs.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Fredrich Dourado Pinto,Bianca Brum de Oliveira,Marcelino Bortolo,Ryan Guldenpfennig,Fábio Ritter Marx,Luciano Trevizan

View all publications

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websiteProtocolBooklet

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Canine IgA ELISA Kit