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AB247192

Bordetella pertussis toxin (PT) IgA ELISA kit

Bordetella pertussis toxin (PT) IgA ELISA kit

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(1 Publication)

Bordetella pertussis toxin (PT) IgA ELISA kit is an indirect ELISA for the qualitative detection of IgA class antibodies against Bordetella pertussis toxin (PT) in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation

別名を表示する

Immunoglobulin alpha-2 heavy chain, Immunoglobulin alpha-2 heavy chain BUT

1 Images
Functional Studies - Bordetella pertussis toxin (PT) IgA ELISA kit (AB247192)
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Supplier Data

Functional Studies - Bordetella pertussis toxin (PT) IgA ELISA kit (AB247192)

Typical Standard Curve – Representative standard curve using ab247192 using the point to point function.

Key facts

検出方法

Colorimetric

サンプルタイプ

Citrate plasma, Heparin Plasma, Serum

交差種

Human

アッセイタイプ

Sandwich

Quantitative
検出範囲

1.56 - 50 IU/mL

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "I-ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Bordetella pertussis toxin (PT) IgA ELISA kit (ab247192) is designed for the quantitative determination of IgA class antibodies against Bordetella pertussis toxin antigens in human serum or plasma (citrate, heparin).

The quantitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.

Bordetella pertussis is a respiratory pathogen that causes pertussis, commonly known as whooping cough, a localized infection of the ciliated epithelium of the bronchial tree. Pertussis is characterized by a prolonged paroxysmal cough often accompanied by an inspiratory whoop. The disease affects mainly children, but adults have also been increasingly reported to be affected. The pathogen produces toxins which cause local damage to the cilia of epithelial cells, which leads to prolonged illness and pertussis. Disease presentation varies with age and history of previous exposure or vaccination. Severe disease is infrequent in healthy, vaccinated persons. Infants, particularly those who have not received the primary vaccination series against pertussis, are at risk for complications and mortality.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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精度

[ { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 12, "mean": "16.29 %", "standardDeviation": null, "coefficientOfVariability": "4.37" }, { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 12, "mean": "6.23 %", "standardDeviation": null, "coefficientOfVariability": "8.17" }, { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 12, "mean": "5.19 %", "standardDeviation": null, "coefficientOfVariability": "7.28" }, { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 23, "mean": "0.547 %", "standardDeviation": null, "coefficientOfVariability": "8.01" }, { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 24, "mean": "1.048 %", "standardDeviation": null, "coefficientOfVariability": "2.66" }, { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 24, "mean": "1.954 %", "standardDeviation": null, "coefficientOfVariability": "2.03" } ]
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製品内容

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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
+4°C
保管に関する情報
+4°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Bordetella pertussis toxin often called pertussis toxin or PTX functions mechanically as an exotoxin produced by Bordetella pertussis. This toxin has a molecular mass of around 105 kDa and comes from the bacterium responsible for whooping cough. It expresses itself in various host cells after the bacterium releases it into the respiratory tract during infection. The pertussis toxin consists of five subunits S1 through S5 which work together to impair host cellular functions by catalyzing the ADP-ribosylation of G-proteins.
Biological function summary

Pertussis toxin disrupts host immune responses and cellular signaling pathways. It is an important component of the ab5 toxin family where one A subunit and five B subunits form a complex to enter cells. The toxin binds to cell surface glycoproteins facilitating its uptake and subsequent inhibition of G-protein-coupled receptor (GPCR) signaling. This action interferes with a variety of intracellular processes ultimately leading to altered immune regulation and enhanced bacterial colonization.

Pathways

Pertussis toxin plays a significant role in the intracellular signaling pathways especially impacting the GPCR pathways. The toxin's catalytic subunit modifies the alpha subunit of inhibitory G-proteins (Gi) which prevents normal signal transduction from the GPCRs. This dysregulation impairs processes such as cyclic AMP (cAMP) regulation and immune response modulation. In the GPCR pathway B. pertussis toxin shows close functional relationships with other G-protein coupled components.

Pertussis toxin significantly contributes to the development of whooping cough a respiratory disease that can lead to severe coughing fits. This toxin affects various host immune proteins like chemokine receptors complicating immune response against the Bordetella pertussis bacterium. Additionally its action on the GPCR signaling pathway implicates it in other related respiratory conditions making pertussis toxin a pivotal target for therapeutic interventions and vaccine development against whooping cough.
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製品プロトコール

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ターゲットの情報

See full target information Bordetella pertussis toxin

追加のターゲット

IGA2_HUMAN
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Multidisciplinary respiratory medicine 17:832 PubMed35865347

2022

A putative link between pertussis and new onset of gastroesophageal reflux an observational study.

Applications

Unspecified application

Species

Unspecified reactive species

Leticia Burton,Daminda P Weerasinghe,David Joffe,Jennifer Saunders,Gregory L Falk,Hans Van der Wall
View all publications
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