ARID1A ELISA Kit (ab302760)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 40.932 pg/ml
- Range: 1718.75 pg/ml - 110000 pg/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Human
製品の概要
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製品名
ARID1A ELISA Kit
ARID1A キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Extract 8 7.4% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Extract 3 8% -
サンプルの種類
Cell culture extracts -
アッセイタイプ
Sandwich (quantitative) -
検出感度
40.932 pg/ml -
検出範囲
1718.75 pg/ml - 110000 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture extracts 103 85% - 114% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse, Rat, Human -
製品の概要
ARID1A SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of ARID1A protein in cell extracts. Quantitate ARID1A with 273 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Sandwich ELISAmore details -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 96 tests 10X ARID1A Capture Antibody 1 x 600µl 10X ARID1A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml ARID1A Lyophilized Recombinant Protein 2 vials Plate Seal 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
研究分野
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機能
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Binds DNA non-specifically. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. -
組織特異性
Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. -
配列類似性
Contains 1 ARID domain. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. - Information by UniProt
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別名
- actin-dependent regulator of chromatin subfamily F member 1
- ARI1A_HUMAN
- ARID domain containing protein 1A
see all -
参照データベース
- Entrez Gene: 8289 Human
- Entrez Gene: 93760 Mouse
- Entrez Gene: 297867 Rat
- Omim: 603024 Human
- SwissProt: O14497 Human
- SwissProt: A2BH40 Mouse
- Unigene: 468972 Human
- Unigene: 22478 Mouse
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab302760の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
|
Sandwich ELISA
Use at an assay dependent concentration. |
画像
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Interpolated concentrations of ARID1A in MCF-7 extract, C2C12 extract, C6 extract.
Interpolated concentration of native ARID1A was measured in duplicate at different sample concentrations. Undiluted samples are as follows: MCF-7 extract 200 µg/mL, C2C12 extract 500 µg/mL, C6 extract 100 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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Example of ARID1A standard curve in 1X Cell Extraction Buffer PTR.Example of ARID1A standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Sandwich ELISA - ARID1A ELISA Kit (ab302760)SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide. -
Benefits of Recombinant Antibodies -
Sandwich ELISA - ARID1A ELISA Kit (ab302760)To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab302760 は論文での使用が確認できていません。