AB315264

3-Nitrotyrosine ELISA Kit - Extracellular

SimpleStep
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3-Nitrotyrosine ELISA Kit - Extracellular is a single-wash 90-min Simplestep used to quantify 3-Nitrotyrosine with a sensitivity of 41 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Competitive ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders

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Competitive ELISA - 3-Nitrotyrosine ELISA Kit - Extracellular (AB315264)

cELISA

Supplier Data

Competitive ELISA - 3-Nitrotyrosine ELISA Kit - Extracellular (AB315264)

Serum of ten individual healthy Human donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean 3-Nitrotyrosine concentration of detected samples was determined to be 183 ng/mL with a range of undetected – 500.7 ng/mL.

cELISA

Supplier Data

Competitive ELISA - 3-Nitrotyrosine ELISA Kit - Extracellular (AB315264)

Linearity of dilution.

Interpolated concentration of native 3-Nitrotyrosine was measured in duplicate at different sample concentrations. Undiluted samples are 50% urine. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

cELISA

Supplier Data

Competitive ELISA - 3-Nitrotyrosine ELISA Kit - Extracellular (AB315264)

Linearity of dilution.

Recombinant 3-Nitrotyrosine was spiked into the following samples and diluted in a 2-fold dilution series in Sample Diluent NS. Undiluted samples are 50% Human serum, plasma (citrate), plasma (EDTA), plasma (heparin), and 25% urine. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

cELISA

Supplier Data

Competitive ELISA - 3-Nitrotyrosine ELISA Kit - Extracellular (AB315264)

Example of 3-Nitrotyrosine standard curve. OD (450 nM) values (mean +/- SD) are graphed

Key facts

検出方法

Colorimetric

サンプルタイプ

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Urine

アッセイタイプ

Competitive

検出感度

= 41 ng/mL

検出範囲

62.5 - 4000 ng/mL

アッセイ時間

1h 30m

アッセイプラットフォーム

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

3-Nitrotyrosine SimpleStep ELISA® kit is a single-wash 90 min competitive ELISA designed for the quantitative measurement of 3-Nitrotyrosine in Serum, Hep Plasma, Cit Plasma, EDTA Plasma, Urine. Quantitate 3-Nitrotyrosine with 41 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture conjugates conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to competitive ELISA allows the formation of the antibody-analyte complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Single-wash protocol reduces assay time to 90 minutes or less
High sensitivity, specificity and reproducibility from superior antibodies
Fully validated in biological samples
96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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精度

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.5" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 9.4" } ]

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回収率

[ { "sample": "Serum", "range": "80 - 89 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "88 - 112 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "83 - 111 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "81 - 100 %", "average": "= 91" }, { "sample": "Urine", "range": "85 - 125 %", "average": "= 101" } ]

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製品内容

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab315264-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB315264-CMP07" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315264-CMP09" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB315264-CMP08" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315264-CMP05" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS", "number":"AB315264-CMP06" }, { "size":"20 x 1 Vial", "name":"3-Nitrotyrosine Lyophilized Standard", "number":"AB315264-CMP03" }, { "size":"10 x 8 mL", "name":"Antibody Diluent 4BI", "number":"AB315264-CMP04" }, { "size":"10 x 80 µL", "name":"50X Nitrotyrosine CaptSure Conjugate", "number":"AB315264-CMP01" }, { "size":"10 x 80 µL", "name":"50X Nitrotyrosine HRP Conjugate", "number":"AB315264-CMP02" }, { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB315264-CMP10" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315264-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315264-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315264-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315264-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315264-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS", "number":"AB315264-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Antibody Diluent 4BI", "number":"AB315264-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"3-Nitrotyrosine Lyophilized Standard", "number":"AB315264-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Nitrotyrosine CaptSure Conjugate", "number":"AB315264-CMP01", "productcode":"" }, { "size":"1 x 80 µL", "name":"50X Nitrotyrosine HRP Conjugate", "number":"AB315264-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB315264-CMP10", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

3-Nitrotyrosine forms when peroxynitrite interacts with tyrosine residues in proteins. It is a marker of nitrosative stress and oxidative damage with the molecular mass of the modified residue remaining similar to that of tyrosine. This molecule is commonly found in conditions with high oxidative stress such as inflammation and is present in tissues with significant reactive nitrogen species. 3-Nitrotyrosine detection is facilitated by various techniques including nitrotyrosine staining and plays a notable role in the study of oxidative stress markers.

Biological function summary

Modification of tyrosine can affect protein function and cell signaling. This post-translational modification can hinder protein function or disrupt protein-protein interactions altering cellular processes. Nitrotyrosine often forms part of the cellular response to oxidative damage contributing to complex antioxidant defenses. The presence of nitrotyrosine indicates a cellular environment stressed by reactive nitrogen species linking it to pathophysiological processes in cells undergoing such stress.

Pathways

3-nitrotyrosine acts in oxidative stress and cell signaling routes. It integrates into pathways related to the cellular stress response particularly the antioxidant defense mechanisms. Proteins such as superoxide dismutase (SOD) may indirectly relate to nitrotyrosine since they mitigate reactive species trying to maintain redox balance. The presence of nitrotyrosine can indicate excess stress on these systems reflecting shifts in signaling that affect cell survival.

High levels of 3-nitrotyrosine associate with cardiovascular diseases and neurodegenerative disorders. In diseases such as atherosclerosis and Alzheimer's 3-nitrotyrosine presence indicates elevated oxidative stress. Through these conditions it relates to proteins like amyloid-beta in Alzheimer's disease or low-density lipoprotein (LDL) in cardiovascular disease. These associations emphasize the potential of 3-nitrotyrosine as a biomarker for oxidative damage in stress-related diseases.

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製品プロトコール

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ターゲットの情報

See full target information 3-Nitrotyrosine

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websiteProtocolBooklet

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