AB116691

3-Nitrotyrosine ELISA Kit

Name: 3-Nitrotyrosine ELISA Kit (ab116691) | Abcam
Brand: Abcam Limited
SKU: AB116691
Price: 101000 JPY
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(77 Publications)

3-Nitrotyrosine ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of 3-Nitrotyrosine in in Cell/Tissue Extracts samples.

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Biochemical assay - 3-Nitrotyrosine ELISA Kit (AB116691)

Biochemical assay

PubMed

Biochemical assay - 3-Nitrotyrosine ELISA Kit (AB116691)

Chen et al used Lipid Peroxidation (MDA) Assay Kit ab118970, 3-Nitrotyrosine Kit ab116691, and 8-OHdG ELISA kit ab201734 to investigate oxidative stress in spinal cord injury in mice.

FGF5 overexpression reduces inflammation and oxidative stress in mice treated with surgery to create spinal cord injury. Yellow = control. Green = FGF5 overexpression. Mice were overexpressed with FGF5 using lentiviral vectors, and then were exposed to SCI or sham surgery 2 weeks post-injection. The levels of MDA, 3-NT and 8-OHdG. n = 6 for each groups. Data are expressed as the mean ± standard deviation and p < 0.05 was considered statistically significant. *p < 0.05.

Levels in the spinal cord of malondialdehyde (MDA), 3-nitrotyrosine (3-NT) and 8-hydroxy 2 deoxyguanosine (8-OHdG) were detected using commercial kits (#ab118970 for MDA, #ab116691 for 3-NT, #ab201734 for 8-OHdG; Abcam) to evaluate oxidative damage of lipids, proteins and nucleic acids according to the manufacturer's instructions.

Sandwich ELISA - 3-Nitrotyrosine ELISA Kit (AB116691)

sELISA

Supplier Data

Sandwich ELISA - 3-Nitrotyrosine ELISA Kit (AB116691)

Example standard curve.

Key facts

検出方法

Colorimetric

サンプルタイプ

Cell culture extracts, Tissue Extracts

アッセイタイプ

Sandwich (quantitative)

検出感度

= 8 ng/mL

検出範囲

8 - 1000 ng/mL

アッセイプラットフォーム

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

ab116691 is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of 3-nitrotyrosine in cell and tissue lysates. The assay employs an antibody specific for 3-nitrotyrosine coated on a 96-well plate. Standards and samples are pipetted into the wells and 3-nitrotyrosine present in the sample is bound to the wells by the immobilized antibody. The wells are washed and a biotin labeled anti-3-nitrotyrosine detector antibody is added. After washing away unbound detector antibody, HRP-conjugated streptavidin specific for the biotin labeled detector antibody is pipetted into the wells. The wells are again washed, an HRP substrate solution (TMB) is added to the wells and color develops in proportion to the amount of 3-nitrotyrosine bound. The developing blue color is measured at 600 nm. Optionally the reaction can be stopped by adding hydrochloric acid which changes the color from blue to yellow and the intensity can be measured at 450 nm.

Store all components at 4°C. This kit is stable for 6 months from receipt. After reconstitution the standard should be stored at -80°C. Unused microplate strips should be returned to the pouch containing the desiccant and resealed.

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精度

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "13" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.9" } ]

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製品内容

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab116691-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"20X Buffer", "number":"AB116691-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"HRP Development Solution", "number":"AB116691-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Microplate 96 antibody coated wells in 12 strips", "number":"AB116691-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"Extraction Buffer (ab260490)", "number":"AB116691-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"10X 3-nitrotyrosine Detector Antibody", "number":"AB116691-CMP07", "productcode":"" }, { "size":"1 x 1 Vial", "name":"3-nitrotyrosine BSA standard (Lyophilized)", "number":"AB116691-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"10X Blocking Buffer", "number":"AB116691-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"10X HRP Label", "number":"AB116691-CMP02", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

+4°C

保管に関する情報

+4°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Nitrotyrosine also known as 3-nitrotyrosine is a marker for nitrosative stress and oxidative damage. It comes from the nitration of tyrosine an amino acid primarily through the action of reactive nitrogen species. Nitrotyrosine can be found in proteins and its molecular mass varies depending on the protein context. It is present across various tissues and fluid samples within the body particularly where high oxidative stress occurs.

Biological function summary

Nitrotyrosine acts as an indicator of cellular damage and protein modification due to stress conditions. When proteins have nitrotyrosine residues their function might change which can lead to cellular signaling disruption. Nitrotyrosine is not usually part of a specific complex but it affects proteins and enzymes by altering their activity and stability contributing to larger stress response mechanisms.

Pathways

Proteins modified by nitrotyrosine are involved in cellular pathways related to oxidative stress and inflammation. Particularly pathways like the nitric oxide synthase pathway and the inflammatory response pathway include nitrotyrosine-modified proteins. It is related to proteins such as nitric oxide synthase (NOS) and superoxide dismutase (SOD) important in managing oxidative balance and stress responses in cells.

Nitrotyrosine is significantly linked to diseases related to oxidative stress such as cardiovascular disease and neurodegenerative disorders. These conditions often have increased levels of nitrotyrosine indicating heightened oxidative damage. Through these diseases nitrotyrosine connects with proteins like amyloid-beta in neurodegenerative disorders and endothelial nitric oxide synthase (eNOS) in cardiovascular diseases reflecting the impact of oxidative stress on these proteins.

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製品プロトコール

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ターゲットの情報

See full target information Nitrotyrosine

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文献 (77)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:467 PubMed40592821

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Current understanding of eryptosis: mechanisms, physiological functions, role in disease, pharmacological applications, and nomenclature recommendations.

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Dysregulated inflammation, oxidative stress, and protein quality control in diabetic HFpEF: unraveling mechanisms and therapeutic targets.

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Cardiovascular diabetology 24:203 PubMed40361188

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Diabetes mellitus aggravates myocardial inflammation and oxidative stress in aortic stenosis: a mechanistic link to HFpEF features.

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Melissa Herwig,Marcel Sieme,Andrea Kovács,Muchtiar Khan,Andreas Mügge,Wolfgang E Schmidt,Ferhat Elci,Shan Sasidharan,Peter Haldenwang,Jan Wintrich,Benjamin Sasko,Ibrahim Akin,Máthé Domokos,Francesco Paneni,Ibrahim El-Battrawy,Zoltán V Varga,Francisca Saraiva,Adelino F Leite-Moreira,Péter Ferdinandy,Loek van Heerebeek,Inês Falcão-Pires,Nazha Hamdani

Microcirculation (New York, N.Y. : 1994) 31:e12888 PubMed39325678

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PDE9A Inhibition Improves Coronary Microvascular Rarefaction and Left Ventricular Diastolic Dysfunction in the ZSF1 Rat Model of HFpEF.

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Journal of extracellular vesicles 13:e12455 PubMed38887871

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Microglial activation induces nitric oxide signalling and alters protein S-nitrosylation patterns in extracellular vesicles.

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Natasha Vassileff,Jereme G Spiers,Sarah E Bamford,Rohan G T Lowe,Keshava K Datta,Paul J Pigram,Andrew F Hill

Molecular neurobiology 61:2357-2366 PubMed37874480

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Electroacupuncture Ameliorates Neuronal Injury by NLRP3/ASC/Caspase-1 Mediated Pyroptosis in Cerebral Ischemia-Reperfusion.

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Cardiovascular diabetology 22:216 PubMed37592255

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Endothelial MICU1 alleviates diabetic cardiomyopathy by attenuating nitrative stress-mediated cardiac microvascular injury.

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Xide Shi,Chao Liu,Jiangwei Chen,Shiqiang Zhou,Yajuan Li,Xingcheng Zhao,Jinliang Xing,Junhui Xue,Fengzhou Liu,Fei Li

Nature communications 14:3383 PubMed37291168

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Tisp40 prevents cardiac ischemia/reperfusion injury through the hexosamine biosynthetic pathway in male mice.

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The European journal of neuroscience 57:1184-1196 PubMed36788114

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Prenatal exposure of diabetes and progestin-mediated autistic biomarker in peripheral blood mononuclear cells.

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The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 27:9-20 PubMed36575929

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Hyperbaric oxygenation applied before or after mild or hard stress: effects on the redox state in the muscle tissue.

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3-Nitrotyrosine ELISA Kit