Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Janus Green cell normalization stain (ab111622)

Submit a review Q&A (2)References (1)

製品の概要

  • 製品名

    Janus Green cell normalization stain

  • アプリケーション

    適用あり: In-Cell ELISAmore details

  • 特記事項

    Ready-to-use green stain for normalizing antibody signal intensity to total cell amounts during in-cell ELISA (ab111622).

    Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain. Incubate cells for 5 minutes at room temperature. Dye should be removed and washed 5 times in ultrapure water or until excess dye is removed. The last wash should be removed and add 0.1 mL 0.5 M HCl per well and incubate for 10 minutes. Shake the plate for 10 seconds, and measure the OD 595 nm using a standard microplate spectrophotometer.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Store at +4°C.

  • バッファー

    Constituents: 0.02% Monobasic dihydrogen potassium phosphate, 0.216% Dibasic monohydrogen sodium phosphate, 0.02% Potassium chloride, 0.81% Sodium chloride, Water, 0.3% Janus Green B
  • Concentration information loading...

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab111622の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
In-Cell ELISA
Use at an assay dependent dilution. 50 µL/ well fixed cells
特記事項
In-Cell ELISA
Use at an assay dependent dilution. 50 µL/ well fixed cells

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab111622 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab111622 は 1 報の論文で使用されています。

  • Liang KX  et al. Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations. EMBO Mol Med 12:e12146 (2020). PubMed: 32840960

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

For this janus green assay, the protocol states to read the plate at 595nM. Can the absorbance of the 0.1 ml HCl solution be read in the same wells the cells were grown or do you recommend spinning down the plate, removing the solution to clean well prior to taking OD measurements?

Read More
Answer

Yes, the OD 595 can be read directly on the plate where the cells were grown and the assay was performed. The HCL will disrupt the cells and the janus green (which stains the nuclei) will be released into the well. No need to spin or move the HCL to a different plate.

Read More

Question

Dear Sirs,
a customer of ours would like to know which is the right wavelenght to read the signal of Janus Green.

Thanks in advance
Kind regards

Read More
Answer

Thank you for your enquiry,

We recommend to measure the assays results at OD595 nm using a standard microplate spectrophotometer. We will be amending the datasheet to make this clearer.

Whole Cell Staining with Janus Green:

1. Completely remove HRP Development Solution from themicroplate wells and blot to dry. Add 50 μl of 1X JanusGreen Stain per well. Incubate plate for 5 minutes atroom temperature.

2. Remove dye, wash plate 5 times in ultrapure water oruntil excess dye is removed.

3. Remove last water wash, blot to dry, add 100 μl of 0.5 MHCl and incubate for 10 minutes.

4. Shake the plate for 10 seconds, and measure the OD595 nm using a standard microplate spectrophotometer

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com