Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
製品の概要
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製品名
Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X)
Annexin V/ANXA5 ihc 試薬 製品一覧 -
アプリケーション
適用あり: FM, Flow Cyt, ICC/IFmore details -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 1001 Annexin V-FITC Reagent.
Annexin V/ANXA5-FITC Apoptosis Detection Reagent ab14082 (previously known as Annexin V-FITC Reagent 1001) can be used in a 10 min, one-step staining procedure to detect apoptosis by staining phosphatidylserine molecules which have translocated to the outside of the cell membrane. Analysis is by flow cytometry or fluorescence microscopy.
This Annexin V/ANXA5-FITC reagent can be used with Annexin V/ANXA5 binding buffer ab14084 (see protocol section for instructions). The Annexin V/ANXA5-FITC reagent is also available as part of Annexin V/ANXA5-FITC apoptosis kit ab14085.
For more apoptosis assays, review the full set of Annexin V/ANXA5 assays, or the apoptosis assay and apoptosis marker guide.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 1% BSA, PBS -
Concentration information loading...
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研究分野
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機能
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
関連疾患
Pregnancy loss, recurrent, 3 -
配列類似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
ドメイン
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻訳後修飾
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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別名
- Anchorin CII
- Annexin 5
- Annexin A5
see all
関連製品
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Related Products
- Propidium Iodide (ab14083)
- 10X Annexin V/ANXA5 Binding Buffer (ab14084)
- Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
- Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
- Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
- Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
- Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14082の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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FM |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent dilution.
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ICC/IF |
Use 1µl for 105 cells.
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特記事項 |
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FM
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
ICC/IF
Use 1µl for 105 cells. |
画像
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab14082 は 18 報の論文で使用されています。
- Rubbino F et al. GPR120 prevents colorectal adenocarcinoma progression by sustaining the mucosal barrier integrity. Sci Rep 12:381 (2022). PubMed: 35013389
- Aljohani AI et al. Upregulation of Cyclin B2 (CCNB2) in breast cancer contributes to the development of lymphovascular invasion. Am J Cancer Res 12:469-489 (2022). PubMed: 35261781
- Wang Y et al. Somatic FOXC1 insertion mutation remodels the immune microenvironment and promotes the progression of childhood acute lymphoblastic leukemia. Cell Death Dis 13:431 (2022). PubMed: 35504885
- Kochetkova EY et al. Approaches and Protocols to Analyze Autophagy and Its Role in Death of Apoptosis-Resistant Senescent Tumor Cells. Methods Mol Biol 2445:139-169 (2022). PubMed: 34972991
- Mygland L et al. Identification of response signatures for tankyrase inhibitor treatment in tumor cell lines. iScience 24:102807 (2021). PubMed: 34337362