10X Annexin V/ANXA5 Binding Buffer (ab14084)
製品の概要
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製品名
10X Annexin V/ANXA5 Binding Buffer
Annexin V/ANXA5 ihc 試薬 製品一覧 -
アプリケーション
適用あり: Flow Cyt, FMmore details -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 1006 10X Annexin V Binding Buffer. 1006-100 is the same size as the 100 ml size of ab14084.
The binding buffer contains optimal concentration of calcium which is required for Annexin v/ANXA5 binding to phosphotidylserine (PS) on the cell surface. This 10X reagent should be diluted to 1X with distilled water (ddH2O) before using in the assay.
This buffer can be used in conjunction with our Annexin V/ANXA5 reagents.
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C. -
バッファー
pH: 7.40
Concentrated x10 -
Concentration information loading...
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研究分野
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機能
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. -
関連疾患
Pregnancy loss, recurrent, 3 -
配列類似性
Belongs to the annexin family.
Contains 4 annexin repeats. -
ドメイン
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
翻訳後修飾
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Information by UniProt
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別名
- Anchorin CII
- Annexin 5
- Annexin A5
see all
関連製品
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Related Products
- Annexin V/ANXA5-FITC Apoptosis Detection Reagent (500X) (ab14082)
- Propidium Iodide (ab14083)
- Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085)
- Annexin V-Cy3 Apoptosis Staining / Detection Kit with SYTOX (ab14144)
- Annexin V/ANXA5-Cy5 Apoptosis Staining / Detection Reagent (ab14147)
- Annexin V-Cy5 Apoptosis Staining / Detection Kit (ab14150)
- Annexin V-EGFP Apoptosis Staining / Detection Kit (ab14153)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14084の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use at an assay dependent concentration.
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FM |
Use at an assay dependent concentration.
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特記事項 |
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Flow Cyt
Use at an assay dependent concentration. |
FM
Use at an assay dependent concentration. |
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab14084 は 5 報の論文で使用されています。
- Orniacki C et al. Y-complex nucleoporins independently contribute to nuclear pore assembly and gene regulation in neuronal progenitors. J Cell Sci 136:N/A (2023). PubMed: 37305998
- Nof E et al. Ventilation-induced epithelial injury drives biological onset of lung trauma in vitro and is mitigated with prophylactic anti-inflammatory therapeutics. Bioeng Transl Med 7:e10271 (2022). PubMed: 35600654
- Kochetkova EY et al. Approaches and Protocols to Analyze Autophagy and Its Role in Death of Apoptosis-Resistant Senescent Tumor Cells. Methods Mol Biol 2445:139-169 (2022). PubMed: 34972991
- Paez-Mayorga J et al. Ponatinib Activates an Inflammatory Response in Endothelial Cells via ERK5 SUMOylation. Front Cardiovasc Med 5:125 (2018). PubMed: 30238007
- Grant R et al. A filtration-based protocol to isolate human Plasma Membrane-derived Vesicles and exosomes from blood plasma. J Immunol Methods 371:143-51 (2011). PubMed: 21741384