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AB312810

iFluor® Red 700 Conjugation Kit

iFluor® Red 700 Conjugation Kit

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iFluor ® Red 700 Conjugation Kit (ab312810) offers several standout features:

- Rapid Conjugation: achieve iFluor® Red 700 labeling in under 2 hours with minimal hands-on time.
- Labels up to 100 µg antibody.
- Versatility: suitable for conjugating antibodies and proteins. iFluor® Red 700 -labeled antibodies can be used immediately in applications such as Flow cytometry, ICC/IF and other fluorescence imaging assays without further purification.
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Flow Cytometry - iFluor® Red 700 Conjugation Kit (AB312810)
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Supplier Data

Flow Cytometry - iFluor® Red 700 Conjugation Kit (AB312810)

Flow cytometric analysis of HL-60 cells incubated with (red) or without (green) anti-human HLA-ABC (W6/32 mAb). Cells were then incubated with goat anti-mouse IgG labeled using the iFluor® 700 Antibody Labeling Kit (ab312810). The fluorescence signal was monitored using ACEA NovoCyte flow cytometer in the APC channel.

Key facts

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製品の詳細

iFluor® Red 700 Conjugation Kit provides a convenient method for labelling small amounts of monoclonal, polyclonal antibodies or other proteins (>10 kDa) with the Red Laser-excitable iFluor® Red 700. This dye is optimized to have minimal fluorescence quenching effect on proteins and nucleic acids, with a fluorescence maxima (Ex/Em) of 693/713 nm respectively. These characteristics make it an excellent replacement for Alexa Fluor 700. iFluor® Red 700 is a stable dye and shows good reactivity and selectivity with protein amino groups.

This conjugation kit has all the essential components to perform two separate labelling reactions with no column purification needed. Each of the two vials of iFluor® Red 700 provided in the kit is optimized for labelling ~50 μg antibody

Impure antibodies or antibodies stabilized with bovine serum albumin (BSA) or gelatin will not be labelled well, Abcam offers a range of purification kits

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製品内容

{ "values": { "100g": { "sellingSize": "100 µg", "publicAssetCode":"ab312810-100ug", "assetComponentDetails": [ { "size":"1 x 2 Vial", "name":"iFluor™ 700", "number":"AB312810-CMP01", "productcode":"" }, { "size":"1 x 20 µL", "name":"Reaction Buffer", "number":"AB312810-CMP02", "productcode":"" }, { "size":"1 x 20 µL", "name":"TQ™-Dyed Quench Buffer", "number":"AB312810-CMP03", "productcode":"" } ] } } }
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出荷温度及び保存条件

出荷温度
Ambient - Can Ship with Ice
短期保存温度
-20°C
長期保存温度
-20°C
保管に関する情報
-20°C
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製品プロトコール

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ターゲットの情報

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websiteProtocolBooklet
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