AB269895

Atto 390 Conjugation Kit (Fast) - Lightning-Link®

Name: Atto 390 Conjugation Kit (Fast) - Lightning-Link® (ab269895) | Abcam
Brand: Abcam Limited
SKU: AB269895
Price: 53000 JPY
Availability: InStock

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(1 Publication)

Atto 390 Conjugation Kit (Fast) - Lightning-Link^® (ab269895) offers several standout features:

- Rapid Conjugation: achieve Atto 390 labeling in under 2 hours with just 30 seconds of hands-on time.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: suitable for conjugating antibodies, proteins, and peptides. Atto 390-labeled antibodies can be used immediately in applications such as Western blot, Flow cytometry, ELISA, and Immunohistochemistry (IHC) without further purification.

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Schematic Diagram - Atto 390 Conjugation Kit (Fast) - Lightning-Link® (AB269895)

Schematic Diagram

Supplier Data

Schematic Diagram - Atto 390 Conjugation Kit (Fast) - Lightning-Link® (AB269895)

This illustration demonstrates a general procedure of how the Lightning-Link®(Fast) labeling technology enables the direct labeling of antibodies or proteins.

Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for just 15 minutes. Please see the ab269895 protocol booklet for more details.

Learn more about our Lightning-Link® conjugation kits here

Flow Cytometry - Atto 390 Conjugation Kit (Fast) - Lightning-Link® (AB269895)

Flow Cyt

PubMed

Flow Cytometry - Atto 390 Conjugation Kit (Fast) - Lightning-Link® (AB269895)

Taichman, Russell S., et al used Atto 390 Conjugation Kit (Fast) - Lightning-Link® (ab269895) as part of examining Axl and Tyro3 expression during experimental prostate cancer (PCa) progression. They used the kit to conjugate Atto 390 to antibodies for use in flow cytometry.
Anti-Axl, anti-Tyro3 and anti-Ki67 antibodies were conjugated to the fluorophores APC-Cy7, PE-Cy5, and Atto390 using our Lightning-Link® Conjugation kits. (A) Experimental model. Human PCa cell lines (PC3Luc, DU145Luc) were implanted s.c. into male SCID mice as a model of a primary (1°) tumor development, and removed after 1 month. At monthly intervals thereafter human PCa cells were identified by anti-HLA staining; proliferative status (Ki67 staining) and Axl or Tyro3 levels were evaluated by FACS. (B) Percent expression of Ki67 by lineage depleted (Lin-) marrow cells or by primary tumor cells at 1 month. (C-D) Percent expression of Axl or Tyro3 by primary tumor cells established with (C) DU145 or (D) PC3 cells or by DTCs recovered from marrow over time.

Image from Taichman, Russell S., et al., PloS one, 8(4): e61873. doi: 10.1371/journal.pone.0061873. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

Other - Atto 390 Conjugation Kit (Fast) - Lightning-Link® (AB269895)

Other

Lab

Other - Atto 390 Conjugation Kit (Fast) - Lightning-Link® (AB269895)

Representative image of ATTO 390 Conjugation Kit (Fast) - Lightning-Link® (ab269895)

Components shown from left to right :

ATTO 390 mix (1 vial for 100 µg, 1 mg)

Modifier reagent (1 vial)

Quencher reagent (1 vial)

Securitainer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the conjugation kit.

Note : Color of the lyophilized material in image may not precisely match the color shade in the actual kit.

Other

Lab

Other - Atto 390 Conjugation Kit (Fast) - Lightning-Link® (AB269895)

Representative image of ATTO 390 Conjugation Kit (Fast) - Lightning-Link® (ab269895)

Components shown from left to right :

ATTO 390 mix (3 vials for 3x 10 µg, 3x 100 µg)

Modifier reagent (1 vial)

Quencher reagent (1 vial)

Securitainer

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the conjugation kit.

Note : Color of the lyophilized material in image may not precisely match the color shade in the actual kit.

Key facts

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Conjugation": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Atto 390 Conjugation Kit / Atto 390 Labeling Kit ab269895 uses a simple and quick process for Atto 390 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides. Learn about our antibody labeling kits and their advantages.

To conjugate an antibody to Atto 390 using this kit:
- add modifier to antibody and incubate for 15 mins
- add quencher and incubate for 5 mins
The Atto 390 conjugated antibody can be used immediately in WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.

Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits. Use the FAQ to learn more about the technology, or about conjugating other proteins and peptides to Atto 390.

Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.

This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link^® Rapid Atto 390 Labeling Kit. 349-0005 is the same as the 100 μg size. 349-0010 is the same as the 3 x 100 ug size. 349-0030 is the same as the 3 x 10 ug size.

Amount and volume of antibody for conjugation to Atto 390

Kit size	Recommended amount of antibody¹	Maximum amount of antibody	Maximum antibody volume²
3 x 10 μg	3 x 10 μg	3 x 20 μg	3 x 10 μL
100 μg	1 x 100 μg	1 x 200 μg	1 x 100 μL
3 x 100 μg	3 x 100 μg	3 x 200 μg	3 x 100 μL

¹ Using the maximum amount of antibody may result in less labeling per antibody.
² Ideal antibody concentration is 1mg/ml. 0.5 - 1 mg/ml can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/ml or < 0.5 mg/ml should be diluted /concentrated.

Buffer Requirements for Conjugation

Buffer should be pH 6.5-8.5.

Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.

50mM / 0.6% Tris¹	0.1% BSA²	50% glycerol
0.1% sodium azide	PBS	Potassium phosphate
Sodium chloride	HEPES	Sucrose
Sodium citrate	EDTA	Trehalose

¹ Tris buffered saline is almost always ≤ 50 mM / 0.6%
² BSA can also interfere with the use of the conjugated antibody in tissue staining.

Incompatible buffer constituents

Thiomerosal	Proclin	Glycine
Arginine	Glutathione	DTT

If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.

Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.

Storing and handling conjugation kits

Lyophilized Lightning-Link^® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

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製品内容

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出荷温度及び保存条件

出荷温度

Ambient - Cannot Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

保管に関する情報

-20°C

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製品プロトコール

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ターゲットの情報

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 8:e61873 PubMed23637920

2013

GAS6 receptor status is associated with dormancy and bone metastatic tumor formation.

Applications

Unspecified application

Species

Unspecified reactive species

Russell S Taichman,Lalit R Patel,Rachel Bedenis,Jingcheng Wang,Savannah Weidner,Taibriana Schumann,Kenji Yumoto,Janice E Berry,Yusuke Shiozawa,Kenneth J Pienta

View all publications

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websiteProtocolBooklet

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Atto 390 Conjugation Kit (Fast) - Lightning-Link®