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AB270709

5’ Feature Barcode 抗体 Conjugation Kit - Lightning-Link® - Oligos 21-30

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30

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5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (ab270709) offers several standout features:

- Rapid Conjugation: achieve oligonucleotide barcode labeling in under 4 hours with just 30 seconds of hands-on time.
- High Efficiency: ensures 100% antibody recovery, meaning no loss of valuable antibodies.
- Versatility: suitable for conjugating antibodies, proteins, and peptides making it suitable for various applications.
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Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (AB270709)
  • Schematic Diagram

Supplier Data

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (AB270709)

This illustration demonstrates a general procedure of how Lightning-Link® labeling technology enables the direct labeling of antibodies or proteins.

Simply pipette your antibody or biomolecule of choice into the vial of a lyophilized mixture containing the label of interest and incubate for around 3 hours. Please see the ab270709 protocol booklet for more details.

Learn more about our Lightning-Link® conjugation kits here

Key facts

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組合せ製品

Sample Prep & Detection Kits

AB102778

Antibody Concentration And Clean-Up Kit

Other - Antibody Concentration And Clean-Up Kit (AB102778)

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Sample Prep & Detection Kits

AB270703

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (AB270703)

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Sample Prep & Detection Kits

AB270705

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (AB270705)

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Sample Prep & Detection Kits

AB201795

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®

ELISA - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (AB201795)

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Sample Prep & Detection Kits

AB102890

HRP Conjugation Kit - Lightning-Link®

Conjugation - HRP Conjugation Kit - Lightning-Link® (AB102890)

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製品の詳細

5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 (ab270709) enables simple and rapid conjugation of oligonucleotide barcodes to antibodies to enable cell surface protein analysis in Multiomic Cytometry on 10x Genomics Chromium Controller. This kit is compatible with Chromium Single Cell Immune Profiling.

The kit includes 10 distinct oligonucleotide barcodes ready to conjugate to 10 μg of antibody each. The protocol involves only a few steps, requires minimal hands-on time and doesn't require any expertise in antibody conjugation or oligonucleotide sourcing.

Please find antibody buffer considerations and compatibility in the protocol booklet. For incompatible buffers and low antibody concentrations, please see our antibody purification and concentration kits.

For conjugating antibodies with additional distinct oligonucleotide barcodes, please see 5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10 (ab270703) and 5' Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (ab270705). 100 μg conjugation kits are available on-demand for each of these 30 oligonucleotide barcodes. For conjugating more than 30 antibodies with distinct oligonucleotide barcodes, please contact us to discuss your requirements.

For oligonucleotide barcode sequence information, please see appendix in the protocol booklet.

Storing and handling conjugation kits

Lyophilized Lightning-Link® components are hygroscopic.

Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.

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製品内容

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出荷温度及び保存条件

出荷温度
Ambient - Cannot Ship with Ice
短期保存温度
-20°C
長期保存温度
-20°C
保管に関する情報
-20°C
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製品プロトコール

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ターゲットの情報

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websiteProtocolBooklet
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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