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Star rating, reviews & references links | レビュー (224) 使用論文 (3592) | レビュー (126) 使用論文 (2272) |
製品の概要 |
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修飾 | Unmodified |
Unmodified |
遺伝子ノックアウト 検証済み |
Knockout Validated |
Knockout Validated |
標識 | Unconjugated |
Unconjugated |
免疫動物 | Rabbit |
Rabbit |
アプリケーション | ICC/IF, IHC-P |
Flow Cyt (Intra), ICC/IF, IHC-P, mIHC, WB |
種交差性 | ||
交差種: | Human, Mouse |
Human, Mouse, Rat |
交差が予測される動物種: | Chinese hamster, Common marmoset, Cow, Dog, Horse, Monkey, Pig, Rabbit, Rat, Sheep, Syrian hamster |
Common marmoset |
免疫原の動物種 | Human |
Human |
特記事項 | Ab15580 is batch tested in ICC/IHC. A variability in IHC-Fr performance can occur with this antibody but we can guarantee consistency in IHC-P. Some customers have successfully used ab15580 in Western Blot. Higher molecular weight proteins like Ki67 may be more difficult to detect in WB. We recommend several potential optimisation steps: loading higher amounts of protein (20 µg and above), using lower percentage gels and/or Tris-Acetate gels, increasing antioxidant to maintain protein reduction, decreasing methanol and increasing SDS in the transfer buffer, and increasing time and voltage of transfer. Larger proteins can be subject to degradation more than smaller proteins so lower molecular weight bands may be present. For further information or support please contact our Scientific Support Team. The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As |
ab16667 was switched from a hybridoma to recombinant production method on 24th October 2019. This product is a recombinant monoclonal antibody, which offers several advantages including:
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents. This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com. |
バッファー | pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. |
pH: 7.20 Preservative: 0.1% Sodium azide Constituents: 1% BSA, PBS |
ポリ/モノ | Polyclonal |
Monoclonal |
アイソタイプ | IgG |
IgG |
リコンビナント | Recombinant antibodies |
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More information |
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