AEC Substrate Kit (ab64252)
製品の概要
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製品名
AEC Substrate Kit -
アプリケーション
適用あり: IHC-Pmore details -
特記事項
AEC substrate kit for use in IHC using HRP / peroxidase detection. Ready to use formulation.
Previously called AEC Substrate System (Ready to Use).
IHC protocol suitable for use with AEC Substrate Kit ab64252:1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate antigen retrieval buffer or enzyme (primary antibody dependent) to treat sections. Wash 3 times in buffer.
3. Add enough hydrogen peroxide blocking solution to cover the sections. Incubate for 10 minutes. Wash 2 times in buffer. If necessary, use avidin biotin blocking.
4. Apply protein block (or normal serum from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in antibody diluent and incubate.
6. Wash 4 times in buffer. Incubate slide with biotinylated secondary antibody (or HRP polymer secondary antibody and skip step 7). Wash 4 times in buffer.
7. Apply streptavidin-HRP and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in DAB substrate or AEC Substrate ab64252 and incubate until desired color is achieved (1-10 mins, recommended 10 mins for AEC). Rinse 4 times in buffer.
9. Add enough drops of hematoxylin to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add mounting medium to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C. -
バッファー
pH: 4.7
Constituents: 0.03% Hydrogen peroxide, 0.1% 3-amino-9-ethyl carbazole, 3% Dimethyl formamide
stabilizers -
Concentration information loading... -
研究分野
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関連性
AEC (3-Amino-9-Ethylcarbazole) is a widely used chromogen for immunohistochemical staining. AEC produces a red end product that is soluble in alcohol and must be used with an aqueous counterstain and mounting media. AEC produces a clean, very intense stain that is free of unwanted background. -
別名
- 3 amino 9 ethylcarbazole
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64252の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
Use at an assay dependent dilution.
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| 特記事項 |
|---|
|
IHC-P
Use at an assay dependent dilution. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Substrate Kit (ab64252)Image from Kryza et al. PLoS One; 11(2) :e0149387. doi: 10.1371/journal.pone.0149387. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Immunostaining using anti-hMMP-9 murine monoclonal antibody.Slides of superficial spread melanoma tumors were incubated with the hMMP-9 antibody (ab58803, 1:300). Alkanie phosphatas esecondary antibody was stained with AEC (3-Amino-9-EthylCarbazole, ab 64252, Abcam, Cambridge) for 15 min and slides were counterstained with hematoxylin for examination.
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Immunohistochemistry (Frozen sections) - AEC Substrate Kit (ab64252)Image from Trus et al., Viruses; 12(5):579. doi: 10.3390/v12050579. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/Immunostaining for TGM2 and SOX2 staining in GSC 158 and GSC 157 cells.(a) Positive TGM2 and negative SOX2 staining in GSC 158 cells. (b) Negative TGM2 and positive SOX2 staining in GSC 157 cells. Cells were incubated with primary monoclonal Abs for 1 h at +37 °C (for SOX2, ab6734, 1/200) following the Lab Vision Ready-To-Use AEC Substrate System (ab64252) according to the manufacturer's instructions.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - AEC Substrate System (Ready to Use) (ab64252)Human colon carcinoma stained with anti-Keratin 20 antibody using AEC Substrate System (Ready to Use) (ab64252).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab64252 は 27 報の論文で使用されています。
- van den Berg MF et al. Whole transcriptome analysis of canine pheochromocytoma and paraganglioma. Front Vet Sci 10:1155804 (2023). PubMed: 37691636
- Ronk AJ et al. A Lassa virus mRNA vaccine confers protection but does not require neutralizing antibody in a guinea pig model of infection. Nat Commun 14:5603 (2023). PubMed: 37699929
- Hwang PY et al. A Cdh3-β-catenin-laminin signaling axis in a subset of breast tumor leader cells control leader cell polarization and directional collective migration. Dev Cell 58:34-50.e9 (2023). PubMed: 36626870
- Tambini MD et al. Aβ43 levels determine the onset of pathological amyloid deposition. J Biol Chem 299:104868 (2023). PubMed: 37257821
- Dicks AR et al. Skeletal dysplasia-causing TRPV4 mutations suppress the hypertrophic differentiation of human iPSC-derived chondrocytes. Elife 12:N/A (2023). PubMed: 36810131