Phalloidin-iFluor 647 Reagent (ab176759)
Key features and details
- Assay type: Cell-based (qualitative)
- Platform: Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Phalloidin-iFluor 647 Reagent
F-actin キット 製品一覧 -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Cell-based (qualitative) -
製品の概要
Phalloidin-iFluor 647 Reagent (ab176759) is one of a series of phalloidin conjugates that bind to actin filaments, also known as F-actin. Phalloidin-iFluor 647 can be detected with a fluorescent microscope at Ex/Em = 650/665 nm.
Phalloidin conjugates are convenient probes for labeling, identifying and quantifying animal or plant actin filaments in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. They can also be used in paraffin-embedded samples that have been de-paraffinized.
Review other popular phalloidin dye conjugates, including Phalloidin-iFluor 488, Phalloidin-iFluor 594, Phalloidin-iFluor 555, and Rhodamine Phalloidin, search the website to see all phallodin conjugates, or read the phalloidin staining protocol.
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特記事項
Staining fixed cell or tissue samples with phalloidin conjugates is very simple; it requires a single 20-90 min incubation with the phalloidin, followed by 3 short wash steps. Phalloidin staining can be combined with antibody-based staining by adding the phalloidin conjugate during either the primary or secondary antibody incubation step.
When used in unfixed samples, phalloidin binding leads to a decrease in the disassociation rate of actin subunits from the ends of actin filaments, essentially stabilizing actin filaments through the prevention of filament depolymerisation.
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試験プラットフォーム
Fluorescence microscope
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 300 tests Phalloidin-iFluor 647 Conjugate 1 x 300 tests -
研究分野
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別名
- actin filament
- f actin
- Filamentous actin
画像
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Functional Studies - Phalloidin-iFluor 647 Reagent (ab176759)Burgoyne, Thomas et al., PloS one?vol. 13,1 e0191048., supplementary Fig 2, doi:10.1371/journal.pone.0191048Fluorescence microscopy (FM) image of laminin-1 and actin (phalloidin) staining.
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CytoPainter Phalloidin-iFluor 647 Reagent (ab176759)Actin filaments staining in HeLa cells. Actin filaments (red) were stained with CytoPainter Phalloidin-iFluor 647 reagent (ab176759). Nuclei were stained with DAPI (blue).
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Structure -
Cytopainter Phalloidin-iFluor 647 Reagent (ab176759)Excitation and emission spectra of phalloidin-iFluor 647 reagent.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (76)
ab176759 は 76 報の論文で使用されています。
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- M'Bana V et al. Plasmodium parasitophorous vacuole membrane-resident protein UIS4 manipulates host cell actin to avoid parasite elimination. iScience 25:104281 (2022). PubMed: 35573190