AB185908

ChIP-Seq High Sensitivity Kit

Name: ChIP-Seq High Sensitivity Kit (ab185908) | Abcam
Brand: Abcam Limited
SKU: AB185908
Price: 213000 JPY
Availability: InStock

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(8 Publications)

ab185908 is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues.

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ChIP - ChIP-Seq High Sensitivity Kit (AB185908)

ChIP

Supplier Data

ChIP - ChIP-Seq High Sensitivity Kit (AB185908)

Sample results.

A ChIP-sequencing library was prepared using ChIP-Seq High Sensitivity Kit (ab185908) from rat heart chromatin and polyclonal antibodies against H3K4me3, H3(K9/14)ac and, H3K27me3. Sequencing was carried out on an Illumina HiSeq2500. Bioinformatics analysis of ChIP-seq is performed utilizing Bowtie and MACS.

ChIP-sequencing - ChIP-Seq High Sensitivity Kit (AB185908)

ChIP-seq

Unknown

ChIP-sequencing - ChIP-Seq High Sensitivity Kit (AB185908)

High sensitive ChIP.

The sheared chromatin isolated from different number of MBD-231 cells was used for ChIP-qPCR analysis of RNA polymerase II enrichment in GAPDH promoters.

Spectrum - ChIP-Seq High Sensitivity Kit (AB185908)

Spectrum

Supplier Data

Spectrum - ChIP-Seq High Sensitivity Kit (AB185908)

Size distribution of library fragments.

Ten nanograms of DNA was ChIPed by RNA polymerase II enrichment and used for DNA library preparation.

Key facts

アッセイ時間

サンプルタイプ

Tissue, Suspension cells, Adherent cells

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ChIP-seq": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

ab185908 is a complete set of reagents required for carrying out a successful ChIP-Seq starting from mammalian cells or tissues. The kit is designed to selectively enrich a chromatin fraction containing specific DNA sequences from various species, particularly mammals, and to prepare a ChIP-Seq library for next generation sequencing using Illumina® platforms such as Illumina® Genome Analyzer II, HiSeq and MiSeq systems. The optimized protocol and components of the kit allow capture of low abundance protein/DNA complexes with minimized non-specific background levels and the ability to construct both non-barcoded (singleplexed) and barcoded (multiplexed) ChIP-Seq libraries quickly with reduced bias.

Starting Materials:
Starting materials can include various tissue or cell samples such as culture cells from a flask or plate, fresh and frozen tissues, etc.

Input Amount of Tissue/Cells:

In general, the amount of cells and tissues for each reaction can be 1 x 10⁵ to 1 x 10⁶ and 5 mg to 50 mg, respectively. For optimal preparation, the input amount should be 4 to 5 x 10⁵cells or 20 to 30 mg tissues so that the amount of DNA enriched from the ChIP reaction can range from at least 1 ng to 100 ng.

Illumina® is a registered trademark of Illumina, Inc.

Protein-DNA interaction plays a critical role for cellular functions such as signal transduction, gene transcription, chromosome segregation, DNA replication and recombination, and epigenetic silencing. Identifying the genetic targets of DNA binding proteins and knowing the mechanisms of protein-DNA interaction on a genome-wide scale is important for understanding cellular processes. Chromatin immunoprecipitation (ChIP) followed by next generation sequencing (ChIP-Seq) offers an advantageous tool for studying genome-wide protein-DNA interactions. It allows for detection that a specific protein binds to specific sequences in living cells. In particular, ChIP antibodies targeted against various transcriptional factors (TF) for genome-wide transcription factor binding site analysis by Chip-Seq is in high demand. Such analysis requires that ChIPed DNA contain minimal background for reliably identifying true TF-enriched regions. Currently used ChIP-Seq methods play an important role in identifying genome-wide protein-DNA interaction. The ChIP-Seq High Sensitivity Kit combines microplate-based ultra ChIP and high sensitive DNA library construction technologies.

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製品内容

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Reverse (20 µM)", "number":"AB185908-CMP28", "productcode":"" }, { "size":"1 x 2 Unit", "name":"8-Well Assay Strips (with Frame)", "number":"AB185908-CMP01", "productcode":"" }, { "size":"1 x 5 µL", "name":"GAPDH Primer - Forward (20 µM)", "number":"AB185908-CMP27", "productcode":"" }, { "size":"1 x 13 µL", "name":"End Polishing Enhancer", "number":"AB185908-CMP20", "productcode":"" }, { "size":"1 x 13 µL", "name":"End Polishing Enzyme Mix", "number":"AB185908-CMP21", "productcode":"" }, { "size":"1 x 160 µL", "name":"2X HiFi PCR Master Mix", "number":"AB185908-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"ChIP Buffer", "number":"AB185908-CMP07", "productcode":"" } ] } } }

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

Multi

長期保存温度

Multi

保管に関する情報

Please refer to protocols

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製品プロトコール

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ターゲットの情報

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

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NRF3 suppresses the malignant progression of TNBC by promoting M1 polarization of macrophages via ROS/HMGB1 axis.

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FASN contributes to ADM resistance of diffuse large B-cell lymphoma by inhibiting ferroptosis via nf-κB/STAT3/GPX4 axis.

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Xing Zhong,Weiwei Zhang,Weiming Zhang,Nasha Yu,Wuping Li,Xiangxiang Song

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WYC-209 inhibited GC malignant progression by down-regulating WNT4 through RARα.

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2022

WDR5 drives the development of cervical squamous cell carcinoma by inducing epithelial-mesenchymal transition and cancer-associated fibroblasts formation.

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Fangli Sun,Linmei Mo,Ying Lan,Qiuping Lu,Nengxian Wu,Honglin Song

Scientific reports 11:21500 PubMed34728675

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Integrative genome-wide analysis of dopaminergic neuron-specific PARIS expression in Drosophila dissects recognition of multiple PPAR-γ associated gene regulation.

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Volkan Yazar,Sung-Ung Kang,Shinwon Ha,Valina L Dawson,Ted M Dawson

Journal of hepatocellular carcinoma 8:333-348 PubMed33977095

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LncRNA-CCDC144NL-AS1 Promotes the Development of Hepatocellular Carcinoma by Inducing WDR5 Expression via Sponging miR-940.

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Yingying Zhang,Hongyu Zhang,Shuhuan Wu

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ChIP-Seq High Sensitivity Kit