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U937 cytoplasmic extract lysate (ab14904)

Submit a review Q&A (1)

製品の概要

  • 製品名

    U937 cytoplasmic extract lysate

  • 特記事項

    Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). 

製品の特性

  • Mycoplasma free

    Yes

  • 製品の状態

    Liquid

  • 保存方法

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • バッファー

    pH: 7.90
    Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
  • Concentration information loading...

  • 研究分野

  • 背景

    The U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab14904 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab14904 は論文での使用が確認できていません。

レビューと Q&A

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Question

I would like to know if this cell line (U937) is an adherent type or not. I am cultivating these cells and would like to infect them with Leishmania parasite to determine their in vitro sensitivity to some antileishmanial drugs. If this cell line in not an adherent type, please advise on how could I make them to be adhered to the culture flasks or testing chamber slides.

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Answer

Thank you for your enquiry. U937 cells grow in suspension. To make them adhere, you can try using poly-lysine, fibronectin or collagen coated plates. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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