製品名T-47D whole cell lysate
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
保存方法Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
Concentration information loading...
背景T47D cells carry receptors for a variety of steroids and calcitonin. They express mutant tumor suppressor protein p53 protein. Under normal culturing conditions, they express progesterone receptor constitutively and are responsive to estrogen. They are able to lose the estrogen receptor (ER) during long-term estrogen deprivation in vitro. Cells are therefore used as a model for studies of drug resistance to tamoxifen in patients with mutant p53 breast tumors.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14899 は 1 報の論文で使用されています。
- Haglund F et al. Prolactin receptor in primary hyperparathyroidism - expression, functionality and clinical correlations. PLoS One 7:e36448 (2012). PubMed: 22606260