T-47D whole cell lysate (ab14899)
製品の概要
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製品名
T-47D whole cell lysate -
特記事項
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
バッファー
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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研究分野
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背景
T47D cells carry receptors for a variety of steroids and calcitonin. They express mutant tumor suppressor protein p53 protein. Under normal culturing conditions, they express progesterone receptor constitutively and are responsive to estrogen. They are able to lose the estrogen receptor (ER) during long-term estrogen deprivation in vitro. Cells are therefore used as a model for studies of drug resistance to tamoxifen in patients with mutant p53 breast tumors.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab14899 は 1 報の論文で使用されています。
- Haglund F et al. Prolactin receptor in primary hyperparathyroidism - expression, functionality and clinical correlations. PLoS One 7:e36448 (2012). PubMed: 22606260