SWAP70 overexpression 293T lysate (whole cell) (ab94234)
製品の概要
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製品名
SWAP70 overexpression 293T lysate (whole cell) -
特記事項
ab94234 is a 293T cell transfected lysate in which Human SWAP70 has been transiently over-expressed using a pCMV-SWAP70 plasmid. The lysate is provided in 1X Sample Buffer.
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
研究分野
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背景
Function: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. Tissue specificity: Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760). Similarity: Contains 1 PH domain. Domain: The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding. PTM: Tyrosine-phosphorylated.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94234の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use at an assay dependent dilution.
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| 特記事項 |
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WB
Use at an assay dependent dilution. |
画像
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SDS-PAGE - SWAP70 overexpression 293T lysate (whole cell) (ab94234)ab94234 at 15µg/lane on an SDS-PAGE gel.
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Western blot - SWAP70 overexpression 293T lysate (whole cell) (ab94234)All lanes : Anti-SWAP70 antibody (ab89532) at 1/500 dilution
Lane 1 :SWAP70 overexpression 293T lysate (whole cell) (ab94234)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab94234 は論文での使用が確認できていません。