Human TMED4 knockout HEK-293T cell lysate
Human TMED4 knockout HEK-293T cell lysate
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TMED4 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
別名を表示する
ERS25, Endoplasmic reticulum stress-response protein 25, GMP25iso, HNLF, Putative NF-kappa-B-activating protein 156, Putative NFkB activating protein HNLF, TMED4_HUMAN, Transmembrane emp24 domain-containing protein 4, Transmembrane emp24 protein transport domain containing 4, p24 family protein alpha-3, p24alpha3
- Sanger seq
Unknown
Sanger Sequencing - Human TMED4 knockout HEK-293T cell lysate (AB258721)
Homozygous : 1 bp insertion in exon 1
製品の詳細
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
製品内容
出荷温度及び保存条件
遺伝子名
遺伝子編集のタイプ
遺伝子編集の方法
ノックアウト検証方法
接合型
出荷温度
短期保存温度
長期保存温度
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The Transmembrane Emp24 Domain-Containing Protein 4 is part of a larger complex essential for the proper sorting and packaging of cargo proteins. By functioning as a scaffold TMED4 assists in maintaining the integrity of protein complexes during their transit through cellular compartments. Its expression is not restricted to a specific cell type; rather it is found ubiquitously across various tissues which suggests an important function in diverse cellular processes.
Pathways
TMED4 functions in the secretory pathway and ER-to-Golgi transport. It is integral in the regulation of vesicle formation budding and fusion events connecting with protein partners such as TMED9. These interactions play a role in processes like protein glycosylation and folding which are vital elements of cellular homeostasis. TMED4's role in these pathways highlights its importance in maintaining cellular function and protein processing.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
組合せ製品
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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