AB257743

Human TIAL1 knockout HeLa cell lysate

Name: Human TIAL1 knockout HeLa cell lysate (ab257743) | Abcam
Brand: Abcam Limited
SKU: AB257743
Price: 114000 JPY
Availability: InStock

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TIAL1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.

別名を表示する

Aging associated gene 7 protein, Nucleolysin TIAR, T cluster binding protein, TCBP, TIA 1 related nucleolysin, TIA-1-related protein, TIA1 cytotoxic granule associated RNA binding protein like 1, TIAR_HUMAN

4 Images

Lab

Western blot - Human TIAL1 knockout HeLa cell lysate (AB257743)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : TIAL1 knockout HeLa cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)
Lane 4 : K-562 cell lysate (20 ug)

ab169547 was shown to specifically react with TIAL1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265793 (knockout cell lysate ab257743) was used. Wild-type and TIAL1 knockout samples were subjected to SDS-PAGE. ab169547 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TIAL1 antibody [EPR11323(B)] (<a href='/products/primary-antibodies/tial1-antibody-epr11323b-ab169547'>ab169547</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TIAL1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TIAL1 knockout HeLa cell line (<a href='/products/cell-lines/human-tial1-knockout-hela-cell-line-ab265793'>ab265793</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

K-562 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 49 kDa

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Sanger Sequencing - Human TIAL1 knockout HeLa cell lysate (AB257743)

Sanger seq

Unknown

Sanger Sequencing - Human TIAL1 knockout HeLa cell lysate (AB257743)

Allele-1 : 1 bp insertion in exon2

Sanger seq

Unknown

Sanger Sequencing - Human TIAL1 knockout HeLa cell lysate (AB257743)

Allele-3 : Insertion of the selection cassette in exon2

Sanger seq

Unknown

Sanger Sequencing - Human TIAL1 knockout HeLa cell lysate (AB257743)

Allele-2 : Insertion of the selection cassette in exon2

Key facts

細胞タイプ

HeLa

生物種

Human

組織

Cervix

ノックアウト検証方法

Sanger Sequencing,Western blot

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.

疾病

Adenocarcinoma

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Reactivity data

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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製品内容

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出荷温度及び保存条件

遺伝子名

TIAL1

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Sanger Sequencing, Western blot

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TIAL1 also known as TIA-1 related protein is a 42 kDa protein that plays a mechanical role in RNA processing. It belongs to the RNA-binding protein family and contains three RNA recognition motifs and a glutamine-rich domain. TIAL1 is widely expressed in various tissues including the spleen thymus and brain. It influences the regulation of mRNA stability and translation specifically recognizing and binding to U-rich elements in the 3’-untranslated regions of target mRNAs.

Biological function summary

TIAL1 influences gene expression by associating with stress granules cytoplasmic aggregates formed in response to cellular stress. This association helps inhibit translation initiation modulating the cellular stress response. TIAL1 is part of a complex with other RNA-binding proteins that integrate signaling events and manage mRNA turnover and stress granule dynamics. Through this interaction it affects apoptosis and contributes to cell survival under stress conditions.

Pathways

Studies demonstrate that TIAL1 plays a role in the apoptotic signaling pathway and the stress response pathway. By interacting with proteins such as eIF2α kinase TIAL1 helps mediate the cellular response to environmental and oxidative stress. These pathways maintain cellular homeostasis and TIAL1 assists in regulating translation during stress ensuring proper cell function.

TIAL1 has relevance in neurodegenerative diseases like Alzheimer's and amyotrophic lateral sclerosis (ALS). The formation of stress granules influenced by TIAL1 is connected to the pathological misfolding of proteins implicated by related proteins such as TDP-43 and FUS. Faulty regulation of these processes may lead to the progression of neurodegenerative conditions highlighting TIAL1's significant role in their development.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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Abcam product promise

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Human TIAL1 knockout HeLa cell lysate