AB263925

Human THY1 (CD90) knockout U-2 OS cell lysate

Name: Human THY1 (CD90) knockout U-2 OS cell lysate (ab263925) | Abcam
Brand: Abcam Limited
SKU: AB263925
Price: 113000 JPY
Availability: InStock

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THY1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included.

別名を表示する

CD90, CD90 antigen, CDw90, FLJ33325, MGC128895, T25, THY1_HUMAN, Theta antigen, Thy 1 T cell antigen, Thy 1 cell surface antigen, Thy 1.2, Thy-1 antigen, Thy-1 membrane glycoprotein, Thy1.1, Thymus cell antigen 1, theta

6 Images

Lab

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (AB263925)

Lane 1 : Wild-type U-2 OS cell lysate 20 ug
Lane 2 : THY1 knockout U-2 OS cell lysate 20 ug
Lane 3 : HepG2 cell lysate 20 ug
Lane 4 : PC-12 cell lysate 20 ug
Lane 5 : Human Brain cell lysate 20 ug
False colour image of Western blot : Anti-CD90 / Thy1 antibody [7E1B11] staining at 1/2000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab181469 was shown to bind specifically to CD90 / Thy1. A band was observed at 35-50 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in Thy1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and Thy1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (<a href='/products/primary-antibodies/cd90-thy1-antibody-7e1b11-ab181469'>ab181469</a>) at 1/2000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

THY1 knockout U-2 OS cell lysate at 20 µg

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell line (<a href='/products/cell-lines/human-thy1-cd90-knockout-u-2-os-cell-line-ab262490'>ab262490</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

PC-12 cell lysate at 20 µg

Lane 5:

Human Brain cell lysate at 20 µg

Predicted band size: 17 kDa

Observed band size: 30-50 kDa,35-50 kDa

false

Lab

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (AB263925)

Western blot : Anti-THY1 antibody [EPR28145-53] (ab307736) staining at 1/5000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab307736 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [EPR28145-53] (<a href='/products/primary-antibodies/cd90-thy1-antibody-epr28145-53-ab307736'>ab307736</a>) at 1/5000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 30 µg

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (ab263925)

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell line (<a href='/products/cell-lines/human-thy1-cd90-knockout-u-2-os-cell-line-ab262490'>ab262490</a>)

Lane 2:

THY1 knockout U-2 OS cell lysate at 30 µg

Lane 3:

Human brain cell lysate at 2 µg

Lane 4:

Human kidney cell lysate at 20 µg

Lane 5:

K562 cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 17 kDa

false

Lab

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (AB263925)

Anti-THY1 antibody [7E1B11] (ab181469) staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab181469 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [7E1B11] (<a href='/products/primary-antibodies/cd90-thy1-antibody-7e1b11-ab181469'>ab181469</a>) at 1/1000 dilution

Lanes 1 - 5:

Western blot at 20 µg

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (ab263925)

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell line (<a href='/products/cell-lines/human-thy1-cd90-knockout-u-2-os-cell-line-ab262490'>ab262490</a>)

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

Predicted band size: 17 kDa

false

Lab

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (AB263925)

Anti-THY1 antibody [EPR3132] (ab92574) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92574 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [EPR3132] (<a href='/products/primary-antibodies/cd90-thy1-antibody-epr3132-ab92574'>ab92574</a>) at 1/1000 dilution

Lanes 1 - 5:

Western blot at 20 µg

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (ab263925)

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell line (<a href='/products/cell-lines/human-thy1-cd90-knockout-u-2-os-cell-line-ab262490'>ab262490</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 17 kDa

false

Lab

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (AB263925)

Anti-THY1 antibody [EPR3133] (ab133350) staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133350 was shown to bind specifically to THY1. A band was observed at 25-37 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in THY1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and THY1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [EPR3133] (<a href='/products/primary-antibodies/cd90-thy1-antibody-epr3133-ab133350'>ab133350</a>) at 1/2000 dilution

Lanes 1 - 5:

Western blot at 20 µg

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (ab263925)

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell line (<a href='/products/cell-lines/human-thy1-cd90-knockout-u-2-os-cell-line-ab262490'>ab262490</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 17 kDa

false

Lab

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (AB263925)

Lane 1 : Wild-type U-2 OS cell lysate 20 μg

Lane 2 : THY1 knockout U-2 OS cell lysate 20 μg

False colour image of Western blot : Anti-CD90 / Thy1 antibody [EPR3132] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92574 was shown to bind specifically to CD90 / Thy1. A band was observed at 35-45 kDa in wild-type U-2 OS cell lysates with no signal observed at this size in Thy1 knockout cell line ab262490 (knockout cell lysate ab263925). To generate this image, wild-type and Thy1 knockout U-2 OS cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 ^°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-CD90 / Thy1 antibody [EPR3132] (<a href='/products/primary-antibodies/cd90-thy1-antibody-epr3132-ab92574'>ab92574</a>) at 1/500 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

Western blot - Human THY1 (CD90) knockout U-2 OS cell lysate (ab263925) at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Lanes 1 - 2:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 35-45 kDa,55 kDa

false

Key facts

細胞タイプ

U-2 OS

生物種

Human

組織

Bone

ノックアウト検証方法

Next Generation Sequencing,Western blot

疾病

Osteosarcoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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製品内容

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263925-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type U-2 OS cell lysate", "number":"AB263925-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human THY1 knockout U2OS cell lysate", "number":"AB263925-CMP02", "productcode":"" } ] } } }

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出荷温度及び保存条件

遺伝子名

THY1

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Next Generation Sequencing, Western blot

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD90 also known as Thy1 is a glycoprotein with a molecular mass of approximately 25-37 kDa. It is highly conserved across species and found on the surface of various cell types including fibroblasts neurons and endothelial cells. CD90 is expressed in these cells in tissues such as the brain thymus and lungs. Researchers use anti-CD90 antibodies for detection often in combination with CD90 FITC in immunofluorescence assays. CD90 serves as a useful cell marker particularly in identifying mesenchymal stem cells (MSCs).

Biological function summary

CD90 plays a role in cell-cell and cell-matrix interactions contributing to cellular adhesion migration and signal transduction. It functions as part of a complex involving integrins which mediate its interaction with the extracellular matrix. This protein also influences T-cell activation reflecting its importance in immune response regulation. Its presence aids in modulating response to injury and promoting tissue repair due to its association with stem and progenitor cells.

Pathways

CD90 engagement affects focal adhesion and MAPK signaling pathways. It interacts with integrins like αvβ3 and α5β1 influencing cell movement and proliferation processes. CD90 involvement in these pathways allows it to regulate responses important for cell survival and communication within various tissues. These interactions highlight the protein's role in maintaining cellular homeostasis and regulating physiological processes.

CD90 has associations with fibrosis and cancer. In fibrotic conditions CD90 expression affects fibroblast activity influencing tissue scarring in organs such as the liver and lungs. In cancer CD90 modifies tumor progression and metastasis by impacting cell adhesion and migration with connections to proteins like TGF-beta which further drive these processes. Understanding CD90 in disease contexts facilitates the development of therapeutic strategies aimed at modulating its activity.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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Abcam product promise

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Human THY1 (CD90) knockout U-2 OS cell lysate