Human SMAD7 (MADH7) knockout HeLa cell lysate
Human SMAD7 (MADH7) knockout HeLa cell lysate
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SMAD7 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.
別名を表示する
CRCS3, FLJ16482, MAD (mothers against decapentaplegic Drosophila) homolog 7, MAD homolog 8, MAD mothers against decapentaplegic homolog 7, MADH 7, MADH 8, MADH6, Mad homolog 7, Mothers Against Decapentaplegic Drosophila Homolog of 6, Mothers Against Decapentaplegic Drosophila Homolog of 7, Mothers against DPP homolog 7, Mothers against DPP homolog 8, Mothers against decapentaplegic homolog 7, Mothers against decapentaplegic homolog 8, SMA- AND MAD-RELATED PROTEIN 7, SMAD, SMAD family member 7, SMAD, mothers against DPP homolog 7, SMAD, mothers against DPP homolog 7 (Drosophila), SMAD7_HUMAN, hSMAD 7
- Sanger seq
Unknown
Sanger Sequencing - Human SMAD7 (MADH7) knockout HeLa cell lysate (AB258686)
Homozygous : 1 bp insertion in exon4
製品の詳細
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
製品内容
出荷温度及び保存条件
遺伝子名
遺伝子編集のタイプ
遺伝子編集の方法
ノックアウト検証方法
出荷温度
短期保存温度
長期保存温度
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SMAD7 functions to regulate the TGF-beta signaling pathway by operating as a negative feedback mediator. It binds to TGF-beta receptors recruiting E3 ubiquitin ligases which promote receptor degradation. SMAD7 is not part of complex large-scale multi-protein assemblies but is essential in modulating the pathway's activity. Its balance controls important processes like cell proliferation differentiation and apoptosis across varied biological contexts.
Pathways
SMAD7 strongly influences the TGF-beta and BMP (bone morphogenetic protein) signaling pathways. SMAD7 blocks TGF-beta signaling by interfering with the activity of R-SMADs such as SMAD2 and SMAD3 and it modulates the BMP pathway by interacting with SMAD1 and SMAD5. Its regulatory roles are tightly integrated within these pathways highlighting its importance in cellular homeostasis and response to extracellular signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
組合せ製品
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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