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AB258647

Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate

Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate

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RTN3 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

別名を表示する

ASYIP, NSP-like protein 2, NSP-like protein II, NSPL2, NSPLII, Neuroendocrine-specific protein-like 2, Neuroendocrine-specific protein-like II, RTN3_HUMAN, Reticulon-3

2 Images
Sanger Sequencing - Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate (AB258647)
  • Sanger seq

Unknown

Sanger Sequencing - Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate (AB258647)

Homozygous : 1 bp insertion in exon 1

Western blot - Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate (AB258647)
  • WB

Lab

Western blot - Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate (AB258647)

False colour image of Western blot : Anti-RTN3/HAP antibody staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab68328 was shown to bind specifically to RTN3/HAP. A band was observed at 24 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in RTN3 knockout cell line ab266517 (knockout cell lysate ab258647). To generate this image, wild-type and RTN3 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-RTN3/HAP antibody (<a href='/products/primary-antibodies/rtn3-hap-antibody-ab68328'>ab68328</a>) at 1/500 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate (ab258647) at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

U-87 MG cell lysate at 20 µg

Predicted band size: 113 kDa

Observed band size: 24 kDa

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Key facts

細胞タイプ

HEK-293T

生物種

Human

組織

Kidney

ノックアウト検証方法

Sanger Sequencing

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

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Reactivity data

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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製品内容

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出荷温度及び保存条件

遺伝子名
RTN3
遺伝子編集のタイプ
Knockout
遺伝子編集の方法
CRISPR technology
ノックアウト検証方法
Sanger Sequencing
接合型
Homozygous
出荷温度
Ambient - Can Ship with Ice
短期保存温度
-20°C
長期保存温度
-20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

RTN3 also known as reticulon-3 or HAP (Hydroxy Alkenal Protection protein) is a member of the reticulon family of proteins which have roles in various cellular processes. This protein has a mass of approximately 130 kDa and is expressed in several tissues with significantly higher levels in the brain and spinal cord. RTN3 localizes to the endoplasmic reticulum where it participates in maintaining the structure of the tubular network influencing intracellular transport and signaling.
Biological function summary

The RTN3/HAP protein can influence neurite outgrowth implicating it in the development and maintenance of the nervous system. This protein associates closely with other members of the reticulon family forming complexes important for its functional roles within the cell. RTN3/HAP has been implicated in mechanisms that protect neurons from stress especially related to endoplasmic reticulum stress by its participation in membrane remodeling and regulation of lipid metabolism.

Pathways

Data shows that RTN3/HAP interacts with components of the endoplasmic reticulum stress response pathway and lipid biosynthesis pathways. It works alongside proteins such as RTN4/Nogo contributing to the regulation of axon guidance and regeneration. These pathways are essential for maintaining cellular homeostasis and responding to physiological stress with RTN3/HAP helping adapt or protect cells under challenging conditions.

Researchers have found associations between RTN3/HAP and neurodegenerative diseases like Alzheimer's disease where it is involved in pathologic processes such as amyloid-beta peptide aggregation. Aberrant expression or mutation of RTN3/HAP can disrupt normal protein turnover linking it to the pathophysiology of such diseases along with related proteins like presenilin and amyloid precursor protein (APP). Understanding these associations offers insights into therapeutic targets for managing these disorders.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

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組合せ製品

Primary Antibodies

AB117819

Anti-ATL3 antibody

Immunoprecipitation - Anti-ATL3 antibody (AB117819)

  • 5
  • 1
Primary Antibodies

AB68328

Anti-RTN3/HAP antibody

KO Validated

Western blot - Anti-RTN3/HAP antibody (AB68328)

  • 3
  • 1
Cell Lines & Lysates

AB266517

Human RTN3 (RTN3/HAP) knockout HEK-293T cell line

Advanced Validation

Western blot - Human RTN3 (RTN3/HAP) knockout HEK-293T cell line (AB266517)

  • 0
  • 0
Proteins & Peptides

AB153066

Recombinant Human RTN3/HAP protein

SDS-PAGE - Recombinant Human RTN3/HAP protein (AB153066)

  • 0
  • 0

Abcam product promise

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