AB258615

Human RAB1A knockout HEK-293T cell lysate

Name: Human RAB1A knockout HEK-293T cell lysate (ab258615) | Abcam
Brand: Abcam Limited
SKU: AB258615
Price: 111000 JPY
Availability: InStock

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RAB1A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 8 bp deletion in exon 1.

別名を表示する

GTP binding protein RAB 1A, RAB 1, RAB1, member RAS oncogene family, RAB1A member RAS oncogene family, RAB1A_HUMAN, Ras-associated protein RAB1, Ras-related protein Rab-1A, YPT1, YPT1-related protein, mKIAA3012

2 Images

Sanger Sequencing - Human RAB1A knockout HEK-293T cell lysate (AB258615)

Sanger seq

Unknown

Sanger Sequencing - Human RAB1A knockout HEK-293T cell lysate (AB258615)

Allele-2 : 8 bp deletion in exon 1

Sanger seq

Unknown

Sanger Sequencing - Human RAB1A knockout HEK-293T cell lysate (AB258615)

Allele-1 : 10 bp deletion in exon 1

Key facts

細胞タイプ

HEK-293T

生物種

Human

組織

Kidney

ノックアウト検証方法

Sanger Sequencing

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 8 bp deletion in exon 1.

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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製品内容

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出荷温度及び保存条件

遺伝子名

RAB1A

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Sanger Sequencing

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Rab1A also known as Ras-related protein Rab-1A is a small GTPase belonging to the Rab family. Rab1A has a role in regulating vesicle trafficking and it is involved in ER-to-Golgi transport. This protein with a molecular mass of approximately 23.5 kDa regulates intracellular traffic affecting vesicle budding motility and fusion. Rab1A is highly expressed in various tissues including the liver heart kidney and to lesser extents in others.

Biological function summary

The Rab1A protein influences intracellular transport processes and maintains cellular homeostasis. It forms part of multimeric protein complexes that facilitate its functions in the secretory pathway. The protein ensures the proper trafficking of cargo proteins from the endoplasmic reticulum to the Golgi apparatus playing a role in the early secretory pathway. This involvement is critical for maintaining the post-translational modification and processing of proteins.

Pathways

The mechanical function of Rab1A integrates into several cellular pathways including the secretory pathway and autophagy. Rab1A works with other proteins like Rab2 and Rab30 in the secretory pathway to coordinate the proper flow of proteins. Its coordination with proteins like LC3 during autophagy highlights its importance in cellular turnover and degradation processes linking Rab1A to essential biological functions and homeostasis.

Rab1A has a connection to certain cancers and neurodegenerative diseases. Aberrant Rab1A expression or mutations can lead to disruptions in cellular trafficking implicated in hepatocellular carcinoma. The protein's altered function could also influence the pathogenesis of Parkinson’s disease with proteins such as α-synuclein showing interaction in disease pathways. Understanding these relationships helps in elucidating Rab1A's role in disease mechanisms.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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Abcam product promise

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Human RAB1A knockout HEK-293T cell lysate