AB257590

Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate

Name: Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (ab257590)
Brand: Abcam Limited
SKU: AB257590
Price: 117000 JPY
Availability: InStock

Be the first to review this product! Submit a review

(0 Publication)

PLCG1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 267 bp deletion in exon1.

別名を表示する

Phospholipase C-II, PLC1, Phospholipase C-gamma-1, PLC-148, PLC-II, PLC-gamma-1, PLCG1, Phosphoinositide phospholipase C-gamma-1

2 Images

Lab

Western blot - Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (AB257590)

Lane 1 : Wild-type HEK-293T cell lysate (40ug)

Lane 2 : PLCG1 knockout HEK-293T cell lysate (40ug)

Lanes 1- 2 : Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - loading control ab8245 observed at 37 kDa.

ab109501 Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] was shown to specifically react with Phospholipase C gamma 1/PLC-gamma-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266530 (knockout cell lysate ab257590) was used. Wild-type and Phospholipase C gamma 1/PLC-gamma-1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^oC at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (<a href='/products/primary-antibodies/phospholipase-c-gamma-1-plc-gamma-1-antibody-epr5358-ab109501'>ab109501</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 40 µg/mL

Lane 2:

PLCG1 knockout HEK-293T cell lysate at 40 µg/mL

Lane 2:

Western blot - Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell line (<a href='/products/cell-lines/human-plcg1-phospholipase-c-gamma-1-plc-gamma-1-knockout-hek-293t-cell-line-ab266530'>ab266530</a>)

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 148 kDa

false

Sanger Sequencing - Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (AB257590)

Sanger seq

Unknown

Sanger Sequencing - Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (AB257590)

Homozygous : 267 bp deletion in exon1

Key facts

細胞タイプ

HEK-293T

生物種

Human

組織

Kidney

ノックアウト検証方法

Sanger Sequencing,Western blot

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, 267 bp deletion in exon1.

style

left-column

style

left-column

製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style

left-column

製品内容

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257590-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257590-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human PLCG1 knockout HEK293T cell lysate", "number":"AB257590-CMP01", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

遺伝子名

PLCG1

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Sanger Sequencing, Western blot

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C gamma 1 (PLC-gamma-1) also known as PLCG1 or PLC-gamma plays an important role in cellular signaling. This enzyme has a molecular mass of approximately 150 kDa. It is found in multiple cell types but is highly expressed in the brain and immune cells. PLC-gamma-1 is involved in the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) to generate inositol 145-trisphosphate (IP3) and diacylglycerol (DAG) leading to downstream signaling events that control various cellular processes.

Biological function summary

PLC-gamma-1 is integral in signal transduction and is an important player in the immune response. The protein does not function as part of a large multi-protein complex but interacts transiently with other signaling molecules. It acts downstream of growth factor receptors and immunoreceptors by mediating calcium release and activation of protein kinase C influencing cell proliferation migration and differentiation. Its expression pattern suggests a critical role in the nervous and immune systems affecting learning memory and immune defense.

Pathways

PLC-gamma-1 is an important component in both the phospholipase C and PLC-gamma pathways. These pathways include important interactions with proteins such as the receptor tyrosine kinases and Src family kinases. In these pathways PLC-gamma-1 modulates signals that guide cellular responses to external stimuli affecting processes like growth signaling and T-cell receptor activation which is vital in adaptive immunity.

PLC-gamma-1 has significant connections to cancer and immune deficiencies. Abnormal PLC-gamma-1 activity is observed in certain cancers such as breast cancer where it influences tumor growth and metastasis. Its dysregulation in immune cells leads to disorders like autoimmune diseases by impacting proteins such as LAT and SLP-76 which are important in T-cell signaling. Understanding PLC-gamma-1’s regulatory mechanisms helps in developing targeted therapies for these conditions.

style

left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style

left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style

left-column

製品プロトコール

Visit the General protocols
Visit the Troubleshooting

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate