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AB258580

Human PI4K2A knockout HeLa cell lysate

Human PI4K2A knockout HeLa cell lysate

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PI4K2A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.

別名を表示する

P4K2A_HUMAN, PI4KII, Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha

2 Images
Sanger Sequencing - Human PI4K2A knockout HeLa cell lysate (AB258580)
  • Sanger seq

Unknown

Sanger Sequencing - Human PI4K2A knockout HeLa cell lysate (AB258580)

Allele-2 : 1 bp deletion in exon1

Sanger Sequencing - Human PI4K2A knockout HeLa cell lysate (AB258580)
  • Sanger seq

Unknown

Sanger Sequencing - Human PI4K2A knockout HeLa cell lysate (AB258580)

Allele-1 : 2 bp deletion in exon1

Key facts

細胞タイプ

HeLa

生物種

Human

組織

Cervix

ノックアウト検証方法

Sanger Sequencing

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.

疾病

Adenocarcinoma

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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製品内容

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出荷温度及び保存条件

遺伝子名
PI4K2A
遺伝子編集のタイプ
Knockout
遺伝子編集の方法
CRISPR technology
ノックアウト検証方法
Sanger Sequencing
出荷温度
Ambient - Can Ship with Ice
短期保存温度
-20°C
長期保存温度
-20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

PI4K2A also known as phosphatidylinositol 4-kinase type 2 alpha is an enzyme involved in phospholipid signaling. It catalyzes the phosphorylation of phosphatidylinositol (PI) to generate phosphatidylinositol 4-phosphate (PI4P). This enzyme is part of the PI4K family and typically has a mass of approximately 55 kDa. PI4K2A is primarily expressed in brain tissue but can also be found in other tissues such as heart and liver.
Biological function summary

PI4K2A plays a significant role in cellular processes through its involvement in phosphoinositide turnover. It contributes to the synthesis of membrane phospholipids and helps regulate vesicle trafficking. PI4K2A functions as part of larger protein complexes involved in generating PI4P a precursor for producing phosphatidylinositol 45-bisphosphate (PI(45)P2) which is critical for various signaling pathways.

Pathways

PI4K2A associates with the phosphoinositide signaling pathway and the endocytic pathway. In the phosphoinositide signaling pathway it interacts with other kinases to facilitate the production of PI4P and PI(45)P2 which serve as signaling molecules. Within these pathways PI4K2A collaborates with proteins like PI4KB to regulate membrane dynamics and endocytosis.

PI4K2A has connections to neurodegenerative diseases and some cardiovascular conditions. Abnormal PI4K2A expression or function may disrupt phosphoinositide balance affecting cellular signaling and leading to disease. It relates to the LRRK2 protein in Parkinson’s disease where dysregulated PI4P levels have implications for disease progression. Furthermore in cardiovascular disorders altered PI4K2A activity may impact heart muscle functionality contributing to disease development.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

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組合せ製品

Cell Lines & Lysates

AB265820

Human PI4K2A knockout HeLa cell line

Sanger Sequencing - Human PI4K2A knockout HeLa cell line (AB265820)

  • 0
  • 0
Proteins & Peptides

AB268860

Recombinant human PI4K2A protein (Active)

Functional Studies - Recombinant human PI4K2A protein (Active) (AB268860)

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Abcam product promise

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