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AB258552

Human OCIAD1 knockout HEK-293T cell lysate

Human OCIAD1 knockout HEK-293T cell lysate

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OCIAD1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.

別名を表示する

Asrij, FLJ20455, MGC111072, OCAD1_HUMAN, OCIA, OCIA domain containing 1, OCIA domain-containing protein 1, Ovarian carcinoma immunoreactive antigen, TPA018

1 Images
Sanger Sequencing - Human OCIAD1 knockout HEK-293T cell lysate (AB258552)
  • Sanger seq

Unknown

Sanger Sequencing - Human OCIAD1 knockout HEK-293T cell lysate (AB258552)

Homozygous : 5 bp deletion in exon 2

Key facts

細胞タイプ

HEK-293T

生物種

Human

組織

Kidney

ノックアウト検証方法

Sanger Sequencing

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.

製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

製品内容

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出荷温度及び保存条件

遺伝子名
OCIAD1
遺伝子編集のタイプ
Knockout
遺伝子編集の方法
CRISPR technology
ノックアウト検証方法
Sanger Sequencing
接合型
Homozygous
出荷温度
Ambient - Can Ship with Ice
短期保存温度
-20°C
長期保存温度
-20°C

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

OCIAD1 also known as ovary carcinoma-associated disintegrin-domain containing protein 1 is a protein with a mass of approximately 35 kDa. It is expressed in various tissues with high levels observed in the ovaries and the central nervous system. Structurally OCIAD1 contains domains that may influence cellular interactions and signal transduction. Studies suggest that OCIAD1 influences cellular processes by participating in membrane-associated complexes which could be important for its mechanical roles.
Biological function summary

OCIAD1 engages in cellular signaling and regulation. It might be a part of a protein complex that contributes to cellular adhesion and proliferation. OCIAD1 appears to support cellular growth and may steer certain cell developmental pathways. By interacting with other proteins it could modulate cellular response mechanisms suggesting its importance in maintaining normal cellular function.

Pathways

OCIAD1 plays roles that impact the PI3K/AKT signaling pathway and the MAPK/ERK pathway. These pathways are essential for the regulation of cell survival growth and differentiation. Within these networks OCIAD1 may interact with proteins like AKT1 and ERK2 facilitating signal transduction that drives cellular responses. Its involvement in these pathways indicates a role in the modulation of critical intracellular signaling events.

OCIAD1 has an established link with ovarian cancer and neurodegenerative diseases such as Alzheimer's disease. In ovarian cancer OCIAD1 might contribute to the malignant transformation and tumor progression possibly in connection with proteins such as p53. In Alzheimer's disease altered OCIAD1 expression could affect proteins like tau which plays a significant role in neurofibrillary tangles. These associations suggest OCIAD1 as a potential target for therapeutic interventions in these conditions.

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

製品プロトコール

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