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AB258044

Human MAT2B (TGR) knockout HEK-293T cell lysate

Human MAT2B (TGR) knockout HEK-293T cell lysate

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MAT2B KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 1 and 5 bp deletion in exon 1.
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Sanger Sequencing - Human MAT2B (TGR) knockout HEK-293T cell lysate (AB258044)
  • Sanger seq

Unknown

Sanger Sequencing - Human MAT2B (TGR) knockout HEK-293T cell lysate (AB258044)

Allele-1 : 16 bp deletion in exon 1

Sanger Sequencing - Human MAT2B (TGR) knockout HEK-293T cell lysate (AB258044)
  • Sanger seq

Unknown

Sanger Sequencing - Human MAT2B (TGR) knockout HEK-293T cell lysate (AB258044)

Allele-2 : 5 bp deletion in exon 1

Key facts

細胞タイプ

HEK-293T

生物種

Human

組織

Kidney

ノックアウト検証方法

Sanger Sequencing

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon 1 and 5 bp deletion in exon 1.

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組合せ製品

Primary Antibodies

AB139132

Anti-MAT2B/TGR antibody

Western blot - Anti-MAT2B/TGR antibody (AB139132)

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Proteins & Peptides

AB89357

Recombinant Human MAT2B/TGR protein (Tag Free)

SDS-PAGE - Recombinant Human MAT2B/TGR protein (Tag Free) (AB89357)

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Cell Lines & Lysates

AB266712

Human MAT2B (TGR) knockout HEK-293T cell line

Sanger Sequencing - Human MAT2B (TGR) knockout HEK-293T cell line (AB266712)

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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製品内容

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出荷温度及び保存条件

遺伝子名
MAT2B
遺伝子編集のタイプ
Knockout
遺伝子編集の方法
CRISPR technology
ノックアウト検証方法
Sanger Sequencing
出荷温度
Ambient - Can Ship with Ice
短期保存温度
-20°C
長期保存温度
-20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

MAT2B also known as TGR is a protein that functions as a regulatory subunit of the enzyme methionine adenosyltransferase II. This enzyme synthesizes S-adenosylmethionine (SAM) essential for methylation reactions in varied cellular processes. MAT2B has a molecular mass of approximately 35 kDa and can modulate the activity of the catalytic subunit of the methionine adenosyltransferase complex. The protein is expressed in multiple tissues with abundant levels in liver and fetal tissues. It is important for regulating the levels of SAM linking dietary methionine to cellular methylation capacity.
Biological function summary

The MAT2B protein helps maintain cellular methylation status by adjusting SAM synthesis. It is a part of the methionine adenosyltransferase II complex which consists of different subunits contributing to its enzymatic activity. The regulatory role of MAT2B ensures precise control over the methionine cycle impacting DNA methylation patterns and gene regulation. By modulating SAM production MAT2B influences cellular growth differentiation and apoptosis through its interactions with other methylation-related proteins.

Pathways

The MAT2B protein plays a significant role in methionine metabolism and the transmethylation pathway. These pathways are central to transferring methyl groups from SAM to a range of substrates including DNA proteins and lipids. MAT2B is related to MAT2A the catalytic subunit that generates SAM. Together they coordinate to balance cellular metabolic needs with the synthesis and utilization of methyl groups securing the methylation-dependent regulation of diverse cellular processes.

MAT2B associates with liver diseases and cancer. Its regulatory function in SAM synthesis ties it to disruptions in methylation capacity observed in these conditions. Reduced MAT2B activity can lead to imbalanced SAM levels affecting the methylation landscape and contributing to altered gene expression involved in tumorigenesis. In liver diseases MAT2B interacts with MAT1A a protein facilitating methylation balance in mature liver tissue. This interaction highlights MAT2B's significant impact on maintaining liver health and its potential role in pathogenesis when dysregulated.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

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Abcam product promise

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