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AB256924

Human FTH1 knockout HEK-293T cell lysate

Human FTH1 knockout HEK-293T cell lysate

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FTH1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 1.

別名を表示する

PIG15, Ferritin heavy chain, OK/SW-cl.84, Cell proliferation-inducing gene 15 protein, FTH, Ferritin H subunit, FTH1, FTHL6

2 Images
Sanger Sequencing - Human FTH1 knockout HEK-293T cell lysate (AB256924)
  • Sanger seq

Unknown

Sanger Sequencing - Human FTH1 knockout HEK-293T cell lysate (AB256924)

Homozygous : 5 bp deletion in exon 1

Western blot - Human FTH1 knockout HEK-293T cell lysate (AB256924)
  • WB

Lab

Western blot - Human FTH1 knockout HEK-293T cell lysate (AB256924)

Lanes 1-3 : Merged signal (red and green). Green - ab75973 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.

ab75973 Anti-Ferritin heavy chain antibody [EPR3004Y] was shown to specifically react with Ferritin heavy chain in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266581 (knockout cell lysate ab256924) was used. Wild-type and Ferritin knockout samples were subjected to SDS-PAGE. ab75973 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (<a href='/products/primary-antibodies/ferritin-heavy-chain-antibody-epr3004y-ab75973'>ab75973</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human FTH1 knockout HEK-293T cell lysate (ab256924) at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 21 kDa,36 kDa

false

Key facts

細胞タイプ

HEK-293T

生物種

Human

組織

Kidney

ノックアウト検証方法

Sanger Sequencing,Western blot

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 1.

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組合せ製品

Cell Lines & Lysates

AB269613

Human ACSL4 (FACL4) knockout A-431 cell line

Advanced Validation

Western blot - Human ACSL4 (FACL4) knockout A-431 cell line (AB269613)

  • 0
  • 0
Primary Antibodies

AB75973

Anti-Ferritin heavy chain antibody [EPR3004Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973)

  • 4
  • 8
Primary Antibodies

AB314553

Anti-NCOA4 antibody [EPR28511-12]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-NCOA4 antibody [EPR28511-12] (AB314553)

  • 0
  • 0
Primary Antibodies

AB69090

Anti-Ferritin Light Chain antibody

Western blot - Anti-Ferritin Light Chain antibody (AB69090)

  • 5
  • 34
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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製品内容

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab256924-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB256924-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human FTH1 knockout HEK293T cell lysate", "number":"AB256924-CMP01", "productcode":"" } ] } } }
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出荷温度及び保存条件

遺伝子名
FTH1
遺伝子編集のタイプ
Knockout
遺伝子編集の方法
CRISPR technology
ノックアウト検証方法
Sanger Sequencing, Western blot
接合型
Homozygous
出荷温度
Ambient - Can Ship with Ice
短期保存温度
-20°C
長期保存温度
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

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Abcam product promise

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