AB256906

Human ENG (CD105) knockout HeLa cell lysate

Name: Human ENG (CD105) knockout HeLa cell lysate (ab256906) | Abcam
Brand: Abcam Limited
SKU: AB256906
Price: 111000 JPY
Availability: InStock

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ENG KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon2 and 19 bp deletion in exon2 and 1 bp insertion in exon2.

別名を表示する

ENG, CD105, Endoglin, END

5 Images

Lab

Western blot - Human ENG (CD105) knockout HeLa cell lysate (AB256906)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : ENG knockout HeLa cell lysate (20 μg)

Lane 3 : HUVEC cell lysate (20 μg)

Lane 4 : MCF7 cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab170943 observed at 70-120 kDa. Red - loading control ab8245 observed at 37 kDa.

ab170943 Anti-CD105 antibody [EPR10145-10] was shown to specifically react with CD105 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab170943 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD105 antibody [EPR10145-10] (<a href='/products/primary-antibodies/cd105-antibody-epr10145-10-ab170943'>ab170943</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ENG knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ENG (CD105) knockout HeLa cell line (<a href='/products/cell-lines/human-eng-cd105-knockout-hela-cell-line-ab265178'>ab265178</a>)

Lane 3:

HUVEC cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 70 kDa

Observed band size: 70-120 kDa

false

Lab

Western blot - Human ENG (CD105) knockout HeLa cell lysate (AB256906)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : ENG knockout HeLa cell lysate (20 μg)

Lane 3 : HUVEC cell lysate (20 μg)

Lane 4 : MCF7 cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab169545 observed at 70-120 kDa. Red - loading control ab8245 observed at 37 kDa.

ab169545 Anti-CD105 antibody [EPR10145-12] was shown to specifically react with CD105 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab169545 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD105 antibody [EPR10145-12] (<a href='/products/primary-antibodies/cd105-antibody-epr10145-12-ab169545'>ab169545</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ENG knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ENG (CD105) knockout HeLa cell line (<a href='/products/cell-lines/human-eng-cd105-knockout-hela-cell-line-ab265178'>ab265178</a>)

Lane 3:

HUVEC cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 70 kDa

Observed band size: 70-120 kDa

false

Sanger Sequencing - Human ENG (CD105) knockout HeLa cell lysate (AB256906)

Sanger seq

Unknown

Sanger Sequencing - Human ENG (CD105) knockout HeLa cell lysate (AB256906)

Allele-2 : 11 bp deletion in exon2

Sanger seq

Unknown

Sanger Sequencing - Human ENG (CD105) knockout HeLa cell lysate (AB256906)

Allele-1 : 19 bp deletion in exon2

Sanger seq

Unknown

Sanger Sequencing - Human ENG (CD105) knockout HeLa cell lysate (AB256906)

Allele-3 : 1 bp insertion in exon2

Key facts

細胞タイプ

HeLa

生物種

Human

組織

Cervix

ノックアウト検証方法

Sanger Sequencing,Western blot

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon2 and 19 bp deletion in exon2 and 1 bp insertion in exon2.

疾病

Adenocarcinoma

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Reactivity data

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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製品内容

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab256906-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human ENG knockout HeLa cell lysate", "number":"AB256906-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB256906-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

遺伝子名

ENG

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Sanger Sequencing, Western blot

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

CD105 also known as endoglin or the CD105 marker is a transmembrane glycoprotein with a molecular weight of approximately 180 kDa. It is a component of the TGF-beta receptor complex and exists in endothelial cells where it is abundantly expressed. Expression of CD105 is higher in proliferating cells particularly in the vasculature. You can also find it in tissues involved in the formation and remodeling of blood vessels such as during angiogenesis.

Biological function summary

Endoglin functions in the regulation of angiogenesis and vascular remodeling. It plays a significant role in mediating cellular responses to TGF-beta signaling influencing endothelial cell proliferation and migration. While not part of a larger structural complex endoglin interacts with receptors and signaling molecules important for vascular development and repair processes. This involvement aids in maintaining endothelial integrity and function under various physiological conditions.

Pathways

CD105 participates in the TGF-beta signaling and angiogenesis pathways. In these pathways it acts in conjunction with other proteins like TGF-beta receptors which play roles in cell differentiation proliferation and apoptosis. The interaction between CD105 and TGF-beta signaling regulates numerous cellular mechanisms impacting angiogenesis and cellular responses to environmental changes.

CD105 has links to hereditary hemorrhagic telangiectasia (HHT) and certain cancers. In HHT mutations in the endoglin gene alter vascular structure leading to the formation of abnormal blood vessels. Oncologically overexpression of CD105 is present in tumor angiogenesis aiding in the progression of certain cancers. Other proteins like VEGF and TGF-beta closely interact with endoglin influencing disease progression and presenting potential targets for therapeutic intervention.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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Abcam product promise

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Human ENG (CD105) knockout HeLa cell lysate