Human EEF1E1 (AIMP3/p18) knockout HEK-293T cell lysate
Human EEF1E1 (AIMP3/p18) knockout HEK-293T cell lysate
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EEF1E1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
別名を表示する
AIMP 3, ARS interacting multifunctional protein 3, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, EEF1E1, Elongation factor p18, Eukaryotic translation elongation factor 1 epsilon-1, Homolog of rat elongation factor p18, MCA3_HUMAN, Multisynthase complex auxiliary component p18, Multisynthetase complex auxiliary component p18, p18, p18 component of aminoacyl tRNA synthetase complex
- Sanger seq
Unknown
Sanger Sequencing - Human EEF1E1 (AIMP3/p18) knockout HEK-293T cell lysate (AB258404)
Homozygous : Insertion of the selection cassette in exon 1
製品の詳細
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
製品内容
出荷温度及び保存条件
遺伝子名
遺伝子編集のタイプ
遺伝子編集の方法
ノックアウト検証方法
接合型
出荷温度
短期保存温度
長期保存温度
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of AIMP3 extends beyond its contribution to protein synthesis. This protein participates in the macromolecular ARS complex which is essential for accurate and efficient translation of the genetic code into functional proteins. AIMP3 acts as a scaffold stabilizing the assembly and function of the ARS complex. Its involvement is critical for maintaining the fidelity of protein synthesis as errors in this process can lead to cellular dysfunction.
Pathways
AIMP3 engages in several key biological processes. It functions within the DNA damage response pathway where it interacts with proteins such as ATM and ATR to facilitate DNA repair mechanisms. Additionally AIMP3 is involved in the p53 signaling pathway where it contributes to the regulation of cell cycle and apoptosis. This interaction highlights the protein's role in maintaining genomic stability and preventing tumorigenesis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
組合せ製品
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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