AB258845

Human DMPK (Dystrophia myotonica protein kinase) knockout HCT116 cell lysate

Name: Human DMPK (Dystrophia myotonica protein kinase) knockout HCT116 cell lysate (ab258845)
Brand: Abcam Limited
SKU: AB258845
Price: 111000 JPY
Availability: InStock

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DMPK KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon1.

別名を表示する

DM, DM 1, DM protein kinase, DM-kinase, DM1 protein kinase, DM1PK, DMK, DMPK_HUMAN, Dystrophia myotonica 1, Dystrophia myotonica protein kinase, MDPK, MT-PK, Myotonic dystrophy associated protein kinase, Myotonic dystrophy protein kinase, Myotonin protein kinase A, Myotonin-protein kinase, Thymopoietin homolog

1 Images

Sanger Sequencing - Human DMPK (Dystrophia myotonica protein kinase) knockout HCT116 cell lysate (AB258845)

Sanger seq

Unknown

Sanger Sequencing - Human DMPK (Dystrophia myotonica protein kinase) knockout HCT116 cell lysate (AB258845)

Homozygous : 1 bp insertion in exon1

Key facts

細胞タイプ

HCT116

生物種

Human

組織

Colon

ノックアウト検証方法

Sanger Sequencing

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon1.

疾病

Carcinoma

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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製品内容

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出荷温度及び保存条件

遺伝子名

DMPK

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Sanger Sequencing

接合型

Homozygous

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Dystrophia myotonica protein kinase also known as DMPK is a serine-threonine kinase with a molecular mass of approximately 69 kDa. The protein localizes mainly in muscle heart and brain tissues serving critical roles in cellular signaling. DMPK influences various cellular processes through its kinase activity modulating several substrates by phosphorylation. This protein is generally expressed in the cytoplasm but can also localize to membranes influencing ion channel activity and cytoskeletal dynamics.

Biological function summary

DMPK acts to regulate muscle function and integrity by modulating phosphorylation states within cells. It is not typically part of known multi-protein complexes but works alongside other kinases to affect signaling pathways. For example DMPK can influence actin filaments and myosin phosphorylation contributing to muscle cell contractility and stability. This regulatory function is essential for the maintenance of normal muscle tone and function.

Pathways

DMPK participates actively in RhoA and MAPK signaling pathways. DMPK impacts the RhoA pathway by regulating cytoskeletal organization and cell morphology interacting with proteins like Rho kinase to modulate muscle contraction. In the MAPK pathway DMPK plays a role in stress response signaling impacting proteins such as ERK that control gene expression in response to stressors.

DMPK is strongly connected to myotonic dystrophy type 1 (DM1) a genetic disorder characterized by muscle weakness and myotonia. Aberrant expression of DMPK due to triplet repeat expansions in its gene leads to toxic RNA accumulation disrupting normal cellular function. This disorder also shares pathogenic features with MBNL1 where both proteins influence RNA-binding and processing contributing to the DM1 phenotype. Understanding the role of DMPK in these conditions may offer insights into potential therapeutic strategies.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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製品プロトコール

Visit the General protocols
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Abcam product promise

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Human DMPK (Dystrophia myotonica protein kinase) knockout HCT116 cell lysate