Human DCAF12 knockout HeLa cell lysate
Human DCAF12 knockout HeLa cell lysate
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DCAF12 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.
別名を表示する
CT102, Centrosome-related protein TCC52, DCA12_HUMAN, DDB1- and CUL4-associated factor 12, DKFZP434O125, KIAA1892, MGC1058, TCC52, Testis cancer centrosome-related protein, WD repeat domain 40A, WD repeat-containing protein 40A, WDR40A, cancer/testis antigen 102
- Sanger seq
Unknown
Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)
Allele-1 : 4 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)
Allele-2 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)
Allele-3 : Insertion of the selection cassette in exon1
製品の詳細
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
製品内容
出荷温度及び保存条件
遺伝子名
遺伝子編集のタイプ
遺伝子編集の方法
ノックアウト検証方法
出荷温度
短期保存温度
長期保存温度
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Abcam product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com