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AB258839

Human DCAF12 knockout HeLa cell lysate

Human DCAF12 knockout HeLa cell lysate

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DCAF12 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.

別名を表示する

CT102, Centrosome-related protein TCC52, DCA12_HUMAN, DDB1- and CUL4-associated factor 12, DKFZP434O125, KIAA1892, MGC1058, TCC52, Testis cancer centrosome-related protein, WD repeat domain 40A, WD repeat-containing protein 40A, WDR40A, cancer/testis antigen 102

3 Images
Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)
  • Sanger seq

Unknown

Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)

Allele-1 : 4 bp deletion in exon1

Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)
  • Sanger seq

Unknown

Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)

Allele-2 : 1 bp deletion in exon1

Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)
  • Sanger seq

Unknown

Sanger Sequencing - Human DCAF12 knockout HeLa cell lysate (AB258839)

Allele-3 : Insertion of the selection cassette in exon1

Key facts

細胞タイプ

HeLa

生物種

Human

組織

Cervix

ノックアウト検証方法

Sanger Sequencing

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.

疾病

Adenocarcinoma

製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

製品内容

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出荷温度及び保存条件

遺伝子名
DCAF12
遺伝子編集のタイプ
Knockout
遺伝子編集の方法
CRISPR technology
ノックアウト検証方法
Sanger Sequencing
出荷温度
Ambient - Can Ship with Ice
短期保存温度
-20°C
長期保存温度
-20°C

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

製品プロトコール

Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

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