AB256857

Human CASP8 (Caspase-8) knockout HeLa cell lysate

Name: Human CASP8 (Caspase-8) knockout HeLa cell lysate (ab256857) | Abcam
Brand: Abcam Limited
SKU: AB256857
Price: 114000 JPY
Availability: InStock

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CASP8 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3.

別名を表示する

MACH, Apoptotic cysteine protease, CAP4, Apoptotic protease Mch-5, FADD-like ICE, FLICE, MCH5, Caspase-8, MORT1-associated ced-3 homolog, FADD-homologous ICE/ced-3-like protease, CASP-8, ICE-like apoptotic protease 5, CASP8

3 Images

Lab

Western blot - Human CASP8 (Caspase-8) knockout HeLa cell lysate (AB256857)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : CASP8 knockout HeLa cell lysate (20µg)

Lane 3 : Jurkat cell lysate (20µg)

Lane 4 : SH-SY5Y cell lysate (20µg)

Lanes 1- 4 : Merged signal (red and green). Green - ab32125 observed at 55 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab32125 Anti-Caspase-8 antibody [E6] was shown to specifically react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32125 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 3000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Caspase-8 antibody [E6] (<a href='/products/primary-antibodies/caspase-8-antibody-e6-ab32125'>ab32125</a>) at 1/3000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CASP8 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CASP8 (Caspase-8) knockout HeLa cell line (<a href='/products/cell-lines/human-casp8-caspase-8-knockout-hela-cell-line-ab264958'>ab264958</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Lab

Western blot - Human CASP8 (Caspase-8) knockout HeLa cell lysate (AB256857)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : CASP8 knockout HeLa cell lysate (20µg)

Lane 3 : Jurkat cell lysate (20µg)

Lane 4 : SH-SY5Y cell lysate (20µg)

Lanes 1- 4 : Merged signal (red and green). Green - ab32397 observed at 55 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab32397 Rabbit monoclonal [EPR2418Y] to IRF3 was shown to specifically react with Caspase-8 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264958 (knockout cell lysate ab256857) was used. Wild-type and Caspase-8 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32397 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 500 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Caspase-8 antibody [E7] (<a href='/products/primary-antibodies/caspase-8-antibody-e7-ab32397'>ab32397</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CASP8 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CASP8 (Caspase-8) knockout HeLa cell line (<a href='/products/cell-lines/human-casp8-caspase-8-knockout-hela-cell-line-ab264958'>ab264958</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

SH-SY5Y cell lysate at 20 µg

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Sanger Sequencing - Human CASP8 (Caspase-8) knockout HeLa cell lysate (AB256857)

Sanger seq

Unknown

Sanger Sequencing - Human CASP8 (Caspase-8) knockout HeLa cell lysate (AB256857)

Homozygous : 2 bp deletion in exon 3

Key facts

細胞タイプ

HeLa

生物種

Human

組織

Cervix

ノックアウト検証方法

Sanger Sequencing,Western blot

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3.

疾病

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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製品内容

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab256857-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB256857-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human CASP8 knockout HeLa cell lysate", "number":"AB256857-CMP01", "productcode":"" } ] } } }

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出荷温度及び保存条件

遺伝子名

CASP8

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Sanger Sequencing, Western blot

接合型

Homozygous

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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Abcam product promise

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保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human CASP8 (Caspase-8) knockout HeLa cell lysate