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AB263099

Human ATP10A knockout A549 cell lysate

Human ATP10A knockout A549 cell lysate

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ATP10A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 4 bp deletion in exon2.

別名を表示する

AT10A_HUMAN, ATP10A, ATP10C, ATPVA, ATPVC, ATPase Class V type 10C, ATPase class V type 10A, ATPase type IV phospholipid transporting (P type), Aminophospholipid translocase VA, KIAA0566, Phospholipid transporting ATPase VA, Probable phospholipid-transporting ATPase VA

2 Images
Sanger Sequencing - Human ATP10A knockout A549 cell lysate (AB263099)
  • Sanger seq

Unknown

Sanger Sequencing - Human ATP10A knockout A549 cell lysate (AB263099)

Allele-1 : 4 bp deletion in exon2

Sanger Sequencing - Human ATP10A knockout A549 cell lysate (AB263099)
  • Sanger seq

Unknown

Sanger Sequencing - Human ATP10A knockout A549 cell lysate (AB263099)

Allele-2 : 1 bp insertion in exon2

Key facts

細胞タイプ

A549

生物種

Human

組織

Lung

ノックアウト検証方法

Sanger Sequencing

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 4 bp deletion in exon2.

疾病

Carcinoma

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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製品内容

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出荷温度及び保存条件

遺伝子名
ATP10A
遺伝子編集のタイプ
Knockout
遺伝子編集の方法
CRISPR technology
ノックアウト検証方法
Sanger Sequencing
出荷温度
Ambient - Can Ship with Ice
短期保存温度
-20°C
長期保存温度
-20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ATP10A also known as ATPase Class V type 10A is a protein involved in phospholipid transport. This protein is part of the P-type ATPase family characterized by its ability to catalyze ATP hydrolysis providing the energy needed for the transport of lipids across cell membranes. ATP10A has an approximate mass of 125 kDa. Expression of ATP10A occurs in various tissues with a higher concentration noted in the brain and adipose tissue.
Biological function summary

ATP10A contributes to maintaining the asymmetrical distribution of phospholipids in the cell membrane which is essential for cell function and integrity. This protein does not function alone; it associates with CDC50 to form a heterodimer complex important for its lipid translocase activity. This activity helps maintain cellular homeostasis impacting processes such as apoptosis and membrane curvature necessary for cell signaling and vesicle formation.

Pathways

The functional role of ATP10A integrates into lipid metabolism and cellular signaling pathways. ATP10A significantly impacts the phospholipid translocation pathway which regulates membrane dynamics and is associated with apoptosis-related proteins like scramblases. Within these pathways ATP10A also interacts with PI3K/AKT a signaling pathway important for cellular growth survival and metabolism.

ATP10A holds importance in neurological and metabolic diseases. Altered expression or mutations in ATP10A appear linked to neurodevelopmental conditions such as Angelman syndrome where an imbalance in phospholipid transport may contribute to pathogenesis. Furthermore ATP10A associates with obesity-related disorders where its role in lipid metabolism links it to proteins like leptin which regulate energy balance and fat storage.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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製品プロトコール

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組合せ製品

Cell Lines & Lysates

AB267040

Human ATP10A knockout A549 cell line

Sanger Sequencing - Human ATP10A knockout A549 cell line (AB267040)

  • 0
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Abcam product promise

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