AB257075

Human ATF3 knockout A549 cell lysate

Name: Human ATF3 knockout A549 cell lysate (ab257075) | Abcam
Brand: Abcam Limited
SKU: AB257075
Price: 114000 JPY
Availability: InStock

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ATF3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp deletion in exon2 and 4 bp deletion in exon2.

別名を表示する

ATF3_HUMAN, ATF3deltaZip2, ATF3deltaZip2c, ATF3deltaZip3, Activating transcription factor 3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3

5 Images

Lab

Western blot - Human ATF3 knockout A549 cell lysate (AB257075)

Lane 1 : Wild-type A549 cell lysate (20 μg)

Lane 2 : ATF3 knockout A549 cell lysate (20 μg)

Lanes 1-2 : Merged signal (red and green). Green - ab207434 observed at 21 kDa. Red - loading control ab8245 observed at 37 kDa.

ab207434 Anti-ATF3 antibody [EPR19488] - ChIP Grade was shown to specifically react with ATF3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266955 (knockout cell lysate ab257075) was used. Wild-type and ATF3 knockout samples were subjected to SDS-PAGE. ab207434 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATF3 antibody [EPR19488] - ChIP Grade (<a href='/products/primary-antibodies/atf3-antibody-epr19488-chip-grade-ab207434'>ab207434</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

ATF3 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human ATF3 knockout A549 cell line (<a href='/products/cell-lines/human-atf3-knockout-a549-cell-line-ab266955'>ab266955</a>)

Predicted band size: 21 kDa

Observed band size: 21 kDa

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Lab

Western blot - Human ATF3 knockout A549 cell lysate (AB257075)

Lane 1 : Wild-type A549 cell lysate (20 μg)

Lane 2 : ATF3 knockout A549 cell lysate (20 μg)

Lanes 1-2 : Merged signal (red and green). Green - ab254268 observed at 21 kDa. Red - loading control ab8245 observed at 37 kDa.

ab254268 Recombinant Anti-ATF3 antibody [EPR22610-19] was shown to specifically react with ATF3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266955 (knockout cell lysate ab257075) was used. Wild-type and ATF3 knockout samples were subjected to SDS-PAGE. ab254268 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATF3 antibody [EPR22610-19] - ChIP Grade (<a href='/products/primary-antibodies/atf3-antibody-epr22610-19-chip-grade-ab254268'>ab254268</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

ATF3 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human ATF3 knockout A549 cell line (<a href='/products/cell-lines/human-atf3-knockout-a549-cell-line-ab266955'>ab266955</a>)

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Sanger Sequencing - Human ATF3 knockout A549 cell lysate (AB257075)

Sanger seq

Unknown

Sanger Sequencing - Human ATF3 knockout A549 cell lysate (AB257075)

Allele-2 : 4 bp deletion in exon2

Sanger seq

Unknown

Sanger Sequencing - Human ATF3 knockout A549 cell lysate (AB257075)

Allele-1 : 14 bp deletion in exon2

Sanger seq

Unknown

Sanger Sequencing - Human ATF3 knockout A549 cell lysate (AB257075)

Allele-3 : 1 bp deletion in exon2

Key facts

細胞タイプ

A549

生物種

Human

組織

Lung

ノックアウト検証方法

Sanger Sequencing,Western blot

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp deletion in exon2 and 4 bp deletion in exon2.

疾病

Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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製品内容

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出荷温度及び保存条件

遺伝子名

ATF3

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Sanger Sequencing, Western blot

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ATF3 also known as Activating Transcription Factor 3 functions primarily as a stress-responsive transcription factor. This protein has a molecular weight of about 21 kDa and is part of the ATF/CREB family of transcription factors. ATF3 is expressed in various tissues and is known for its role in stress response and regulation of gene expression. The expression levels change in response to cellular stress and it may interact with several other transcription factors to modulate gene expression profiles.

Biological function summary

ATF3 acts as a regulator of cellular stress responses and can influence cell cycle arrest and apoptosis. It does not form a large complex but interacts with diverse partners to execute its functions. Studies often use HCT116 cells to investigate ATF3's role where it can serve as a marker of cellular stress and inflammation. The protein regulates genes involved in maintaining homeostasis in stressed cells.

Pathways

ATF3 is involved in stress response and apoptotic pathways. In the context of apoptosis ATF3 interacts with proteins like p53 and Jun which are important for inducing cell cycle arrest and programmed cell death. In stress response pathways ATF3 modifies transcriptional activity in reaction to various stress signals providing a checkpoint for damaged cells before repairing or entering programmed death.

ATF3 relates to cancer and cardiovascular diseases. In cancer abnormal expression of ATF3 can influence tumor growth and metastasis often acting through its interactions with proteins like NF-kB. Additionally in cardiovascular disorders ATF3's role in regulating stress responses underpins its involvement in conditions linked to inflammation and atherosclerosis again with NF-kB signaling being a relevant pathway of interaction. Researchers continue to study ATF3 to uncover therapeutic targets for these and other diseases.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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Abcam product promise

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Human ATF3 knockout A549 cell lysate