AB257250

Human ARID1A knockout HEK-293T cell lysate

Name: Human ARID1A knockout HEK-293T cell lysate (ab257250) | Abcam
Brand: Abcam Limited
SKU: AB257250
Price: 111000 JPY
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

ARID1A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon1.

別名を表示する

B120, hOSA1, BAF250, C1orf4, BRG1-associated factor 250a, SWI/SNF complex protein p270, Osa homolog 1, hELD, OSA1, BRG1-associated factor 250, ARID domain-containing protein 1A, SWI-like protein, AT-rich interactive domain-containing protein 1A, SMARCF1, ARID1A, BAF250A

2 Images

Sanger Sequencing - Human ARID1A knockout HEK-293T cell lysate (AB257250)

Sanger seq

Unknown

Sanger Sequencing - Human ARID1A knockout HEK-293T cell lysate (AB257250)

Homozygous : 11 bp deletion in exon1

Lab

Western blot - Human ARID1A knockout HEK-293T cell lysate (AB257250)

Lanes 1 - 3 : Merged signal (red and green). Green - ab182560 observed at 270 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab182560 was shown to react with ARID1A in wild-type HEK-293T cells in Western blot with loss of signal observed in ARID1A knockout cell line ab266189 (ARID1A knockout cell lysate ab257250). Wild-type HEK-293T and ARID1A knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab182560 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ARID1A antibody [EPR13501] (<a href='/products/primary-antibodies/arid1a-antibody-epr13501-ab182560'>ab182560</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human ARID1A knockout HEK-293T cell lysate (ab257250) at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Lanes 1 - 3:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Predicted band size: 242 kDa

Observed band size: 270 kDa

false

Key facts

細胞タイプ

HEK-293T

生物種

Human

組織

Kidney

ノックアウト検証方法

Sanger Sequencing,Western blot

ノックアウト変異

Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon1.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Sanger seq": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

製品の詳細

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style

left-column

製品内容

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257250-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human ARID1A knockout HEK293T cell lysate", "number":"AB257250-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257250-CMP02", "productcode":"" } ] } } }

style

left-column

出荷温度及び保存条件

遺伝子名

ARID1A

遺伝子編集のタイプ

Knockout

遺伝子編集の方法

CRISPR technology

ノックアウト検証方法

Sanger Sequencing, Western blot

出荷温度

Ambient - Can Ship with Ice

短期保存温度

-20°C

長期保存温度

-20°C

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

ARID1A also known as BAF250A is a protein encoded by the ARID1A gene and forms part of the SWI/SNF chromatin remodeling complex. The molecular weight of ARID1A is approximately 270 kDa. This protein is expressed in many tissues but higher expression is noted in the liver kidney and placenta. ARID1A contains an ARID (A-T Rich Interaction Domain) which allows it to bind DNA influencing transcription regulation significantly.

Biological function summary

ARID1A impacts cellular functions through its role in the SWI/SNF complex. This complex modulates chromatin structure and is integral to DNA accessibility which in turn controls gene expression. ARID1A's involvement helps in regulating transcription factors and affects processes such as cell growth and differentiation. Its function is necessary for maintaining proper cell proliferation and apoptosis balance.

Pathways

ARID1A plays a central role in transcriptional regulation and cell cycle control. It shows interaction with the tumor suppressor protein p53 and is implicated in the PI3K/AKT signaling pathway. ARID1A's function in these pathways affects cellular responses to DNA damage and growth signals linking it closely to important cellular fate decisions.

Mutations or loss of function in ARID1A has been strongly associated with various cancers including ovarian and endometrial cancers. In these contexts ARID1A dysfunction often occurs alongside other proteins such as p53 contributing to tumorigenesis by deregulating tumor suppressive pathways. It also holds a potential connection to neurodevelopmental disorders due to its role in chromatin remodeling and gene expression.

style

left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style

left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style

left-column

製品プロトコール

Visit the General protocols
Visit the Troubleshooting

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed37627124

2023

Development of an Enzyme-Linked Immunosorbent Assay (ELISA) for the Quantification of ARID1A in Tissue Lysates.

Applications

Unspecified application

Species

Unspecified reactive species

Manuel Hinsberger,Julia Becker-Kettern,Wiebke M Jürgens-Wemheuer,Joachim Oertel,Walter J Schulz-Schaeffer

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human ARID1A knockout HEK-293T cell lysate