AB7914

HL-60 whole cell lysate

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(4 Publications)

HL-60 whole cell lysate suitable for WB. View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.

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Project1671****

Western blot - HL-60 whole cell lysate (AB7914)

ab26483 consistently detects a band at 55-60 kDa but we also see bands of ~35 and 70 kDa that appear with varying intensity in some of our extracts. We cannot find an explanation for this and are unsure of the nature of these crossreacting bands.

All lanes:

Western blot - Anti-Cdc20 antibody (<a href='/products/primary-antibodies/cdc20-antibody-ab26483'>ab26483</a>) at 1 µg/mL

Lane 1:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (ab7908) at 20 µg

Lane 2:

Western blot - THP-1 whole cell lysate (<a href='/products/cell-lysates/thp-1-whole-cell-lysate-ab7913'>ab7913</a>) at 20 µg

Lane 3:

Western blot - HEK-293 whole cell lysate (<a href='/products/cell-lysates/hek-293-whole-cell-lysate-ab7902'>ab7902</a>) at 20 µg

Lane 4:

Western blot - HL-60 whole cell lysate (ab7914) at 20 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 55 kDa

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Key facts

細胞タイプ

HL-60

生物種

Human

製品の状態

Liquid

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>HL60 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.</p>" } } }

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製品の詳細

Cell line: HL60 (Human promyelocytic leukemia).
Growth media: RPMI and 10% FBS (Fetal bovine serum).

HL60 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (0.045 M Tris-HCl pH 6.8, 10% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue), containing 0.05 M DTT.

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出荷温度及び保存条件

出荷温度

Blue Ice

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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Cell culture

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製品プロトコール

Visit the General protocols
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39273253

2024

Influence of Anesthetic Regimes on Extracellular Vesicles following Remote Ischemic Preconditioning in Coronary Artery Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Phuong N V Pham,Loubna Yahsaly,Crista Ochsenfarth,Bernd Giebel,Romina Schnitzler,Peter Zahn,Ulrich H Frey

Cells 12: PubMed37887347

2023

Influence of the Anesthetic Technique on Circulating Extracellular Vesicles in Bladder Cancer Patients Undergoing Radical Cystectomy: A Prospective, Randomized Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Luisa Gluth,Crista Ochsenfarth,Phuong Nam Viet Pham,Jan M Wischermann,Thomas Komanek,Florian Roghmann,Ulrich H Frey

PloS one 18:e0286278 PubMed37874822

2023

Interaction of amisulpride with GLUT1 at the blood-brain barrier. Relevance to Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sevda T Boyanova,Ethlyn Lloyd-Morris,Christopher Corpe,Khondaker Miraz Rahman,Doaa B Farag,Lee K Page,Hao Wang,Alice L Fleckney,Ariana Gatt,Claire Troakes,Gema Vizcay-Barrena,Roland Fleck,Suzanne J Reeves,Sarah A Thomas

American journal of respiratory cell and molecular 40:200-10 PubMed18703796

2008

Regulation of bacteria-induced intercellular adhesion molecule-1 by CCAAT/enhancer binding proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Lori J Manzel,Cecilia L Chin,Mark A Behlke,Dwight C Look

View all publications

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HL-60 whole cell lysate