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AB14658

HeLa whole cell lysate (TNF alpha stimulated)

HeLa whole cell lysate (TNF alpha stimulated)

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HeLa whole cell lysate (TNF alpha stimulated). View our extensive range of validated lysates from normal and diseased human, mouse and rat tissue.
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Functional Studies - HeLa whole cell lysate (TNF alpha stimulated) (AB14658)
  • FuncS

Unknown

Functional Studies - HeLa whole cell lysate (TNF alpha stimulated) (AB14658)

ab14658 has been tested for NFkappaB activation. The signal intensity for NFkappaB activation is compared to the signal intensity obtained with extracts from unstimulated HeLa cells. After the signals are blanked, the ratio of the signals from stimulated cells over unstimulated cells must be above 4. This ratio may vary depending on the basal level of NFkappaB activation in a given cell type.

Key facts

細胞タイプ

HeLa

生物種

Human

組織

Cervix

製品の状態

Liquid

form

疾病

Adenocarcinoma

製品の詳細

The HeLa whole cell extracts were collected in Lysis Buffer after a 30 minute incubation with or without TNF alpha (20 ng/ml). The protein content was determined by a Bradford based assay. We recommend aliquoting the extracts into single use fractions and then storing them.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

製品プロトコール

Abcam product promise

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